摘要
目的 以大肠埃希菌不耐热肠毒素B亚单位为佐剂研究汉坦病毒经不同黏膜途径免疫的效果。方法 以乳糖为诱导剂，在大肠埃希菌表达不耐热肠毒素B亚单位（LTB），Ni^2＋亲和层析进行纯化。以灭活汉坦病毒84Fii株为疫苗，LTB为佐剂，分别采用滴鼻、口服、经阴道3种黏膜途径接种C37BL／6小鼠。ELISA检测血清中特异IgG和阴道冲洗液中特异IgA。结果 免疫印迹和神经节苷脂结合活性研究，证实了LTB的表达。经滴鼻、口服、阴道免疫，均可诱导分泌型IgA抗体和血清IgG抗体反应，不同途径接种组间差异无统计学意义。结论 以LTB为佐剂的灭活汉坦病毒通过3种黏膜途径均能产生抗汉坦病毒黏膜免疫和系统免疫应答。
Objective To evaluation the effect of different mucosal vaccination pathway on hantavirus with the recombinant E. coli heat-labile enterotoxin B subunit（rLTB） as adjuvant. Methods The rLTB was expressed and purified. Take the inactivated hantavirus strain 84Fli as vaccine, and immunized C57 BL/6 mice through intranasal, oral and vaginal respectively. Specific IgG and sectory IgA were detected by ELISA in serum, and vaginal washing samples respectively. Results The rLTB was efficiently expressed under the induction of lactose, identified by western blotting and GM-1 binding experiment. The vaccination through intralnasal, oral and vaginal, can induce IgG and sectory IgA response. Conclusion Inactivated hantavirus can produce mucosal immune resoonse with rLTB as adjuvants through intralnasal, oral and vaginal vaccination resoectivelv.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2008年第3期174-176,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
汉坦病毒
免疫
黏膜
免疫接种
Hantan virus
Immunity, mucosal
Vaccination
