摘要
目的探讨甲基强的松龙(methylprednisolone,MP)对大鼠实验性自身免疫性脑脊髓炎(experimental autoimmuneen cephalomyelitis,EAE)新发现的IL-23/IL-17炎性轴发病机制的影响。方法建立Wistar大鼠EAE动物模型,给予糖皮质激素MP和生理盐水预处理建立MP组和EAE组,并设立正常对照组,RT-PCR法检测大鼠脑组织中白介素-12(interleukin-12,IL-12)和白介素-23(interleukin-23,IL-23)亚基IL-23P19、IL-12P40、IL-12P35及白介素-17(interleukin-17,IL-17)前体毒性T细胞相关抗原8(cytotoxic T lymphocyte-associated antigen8,CTLA-8)的mR-NA表达,用ELISA法检测IL-17水平。结果MP组大鼠脑组织中IL-23P19、IL-12P40、IL-12P35及CTLA-8的mR-NA的表达比EAE组低(P<0.05),IL-17水平也低于EAE组(P<0.05),差异均有统计学意义。结论甲基强的松龙能够抑制IL-23/IL-17轴而起到抗炎治疗作用。
Objective To explore the effects of methylprednisolone on newly IL-23/IL-17 immune axis of rats with acute experimental autoimmune encephalomyelitis （EAE）, Methods EAE model was induced in wistar rats,and were respectively treated with methylprednisolone and normal saline as MP group and EAE group. Normal rats were selected as control group, levels of IL-23P19, IL-12P40, IL-12P35, and CTLA-8 mRNA in brain tissue were detected by RT-PCR. The IL-17 level in the brain tissue supernatant was measured by the ELISA method. Results IL-23P19, IL-12P40, IL- 12P35 and CTLA-8 mRNA levels and IL-17 protein levels in brain tissue were significantly decreased in MP treated EAE rats than those in EAE rats （P 〈0. 05）. Conclusions Methylprednisolone may exert anti-inflammatory effect through inhibiting IL-23/IL-17 axis.
出处
《中国神经精神疾病杂志》
CAS
CSCD
北大核心
2008年第6期345-348,共4页
Chinese Journal of Nervous and Mental Diseases
