摘要
以PEG为乳剂，建立了一种新的液相压电免疫分析法，并用于日本血吸虫感染兔血清的测定．初步探讨了这种PEG液相压电免疫传感器的响应机理，详细考察了PEG浓度、PEG－AWA复合时间及测试温度等因素对频移的影响．对5个兔血样的测试结果表明，应用该方法不仅对日本血吸虫病的阳性判别率可达100％，而且能定量测定感染血清中血吸虫抗体的含量．
A new liquid piezoelectric immunoassay was developed to determine infectionrabbit serum (IRS) by adult worm antigen (AWA ) of S. japonicum, which is based on thetination of antibody and antigen-PEG complex by an immunoreaction. By examining thesurface appearances of the quartz crystal with scanning electronic microscopy(SEM) technologyand investigating the effect of the density and viscousity of the solution, it can be found thatthe absorption on the surfaces of the quartz crystal plays a dominant role in the frequencyresponse in the immunoassay. The influences including PEG concentration, complexing time ofAWA-PEG complex and detection temperature on frequency responses were investigated indetail, respectively. The frequency shift was linear with the antibody concentration in therange of 3.6~42.0μg/mL. Five rabbit serum specimens were analyzed with this method andthe analytical results given by this approach show that the detection ratio of positive serumreaches 100% and the antibody concentration can be quantitatively determined.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1997年第11期1774-1778,共5页
Chemical Journal of Chinese Universities
基金
世界卫生组织资助
高等学校博士点基金
湖南省自然科学基金
