摘要
对７０例外阴病变的病例进行严格质控的ＰＣＲ检测，并结合病史、组织病理改变及ＨＰＶ核壳抗原免疫组化检测进行综合分析。结果表明，４０例具有ＣＡ组织病理特征的病例中，３７例ＨＰＶ６／１１ＤＮＡＰＣＲ检测阳性，３０例原病理诊断为鳞状上皮乳头状增生或假性湿疣的病例中，有７例检出ＨＰＶＤＮＡ。综合分析的结果提示，对于有典型ＣＡ组织学特征者，结合临床，即使不做ＰＣＲ检则也可做出正确诊断。ＰＣＲ检测的意义在于为无典型ＣＡ组织学特征的病例增加一有价值的诊断依据，而不能仅根据ＰＣＲ结果武断地做出结论。
To asertain the significance of diagnosis of HPV infection in condylomata acuminate (CA)by PCR technique ,we examined 70 cases with lesions of vulva for the HPV 6/11 infection by strict quality control PCR.The presence of HPV 6/11 DNA in the cases were analyzed synthetically with the case history,histopathological features and HPV-Ag.Of the 40 cases with typical histopathological picture of CA,37(92.5%) cases were positive for HPV 6/11 DNA,HPV DNA was also detected in 7 of 30 cases with papilloma of vulva or pseudocondyloma of vulva .The result of synthetical analyse suggest that it is unlikely necessary to use PCR technique in the cases with typical histopathological changes of CA.The significance of PCR detection lies in providing a valuable diagnosis evidence especialy in some cases with atypical histopathological features.
出处
《中国性病艾滋病防治》
1997年第4期170-172,共3页
Chinese Journal of Std & Aids Prevention and Control
基金
山东省自然科学基金
关键词
尖锐湿疣
PCR
诊断
condylomata acuminata
polymerase chain reaction
effect evaluation
