摘要
目的:研究多房棘球绦虫重组Bb-EmⅡ/3-Em14-3-3疫苗对原头蚴攻击小鼠细胞因子变化的影响。方法:将重组Bb-EmⅡ/3-Em14-3-3疫苗分别通过皮下注射、肌肉注射、鼻腔黏膜接种和口服接种免疫BALB/c小鼠12周后,用50个多房棘球绦虫原头蚴腹腔注射进行攻击,攻击感染后18周剖杀小鼠,取脾脏制备淋巴细胞悬液体外培养,分别以多房棘球绦虫抗原(EmAg)、刀豆素A(ConA)刺激诱生白细胞介素12(IL-12)、白细胞介素10(IL-10)和干扰素γ(IFN-γ);以脂多糖(LPS)刺激诱生肿瘤坏死因子α(TNF-α)。常规ELISA测定脾细胞培养上清液中IL-12、IL-10、IFN-γ和TNF-α水平。结果:与对照组相比,疫苗免疫组IFN-γ、IL-12、TNF-α水平增加,IL-10水平降低,EmAg刺激组和ConA或LPS刺激组细胞因子水平明显高于相应的原液组。结论:多房棘球绦虫重组Bb-EmⅡ/3-Em14-3-3疫苗可诱导攻击感染小鼠产生Th1型细胞免疫应答,增强宿主抗多房棘球绦虫感染的保护性免疫反应。
AIM： To study the effects of recombinant Bb-EmⅡ/3-Em14-3-3 vaccine on cytokine secretion in mice challenged with protoscoleces of Echinococcus multilocularis.METHODS： BALB/c mice were immunized with rBb-EmⅡ/3-Em14-3-3 vaccine by subcutaneous injection,intramuscular injection,nasal mucosa inoculation or oral administration.After 12 weeks of immunization,all the mice were challenged with 50 protoscoleces of Echinococcus multilocularis by intraperitoneal injection.18 weeks later,the mice were sacrificed and the splenocytes were stimulated with EmAg and ConA or LPS in vitro,then IL-12,IL-10,IFN-γ and TNF-α in the culture supernatant were measured by ELISA.RESULTS： The level of IFN-γ,IL-12 and TNF-α were greatly higher than that of PBS control group（P〈0.05 or P〈0.01）,and the level of IL-10 was lower.The level of IFN-γ,IL-12,TNF-α and IL-10 in each group with EmAg and ConA or LPS stimulation was greatly higher than that in the group without stimulation（P〈0.05 or P〈0.01）.CONCLUSION： Th1 response is induced in mice challenged with Echinococcus multilocularis by rBb-EmⅡ/3-Em14-3-3 vaccine,and the vaccine may enhance protective response against the challenge with protoscoleces of Echinococcus multilocularis.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2008年第8期781-784,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(30500423)
教育部重点项目资助(205131)
重庆市卫生局科研基金资助项目(05-2-203)
