摘要
[目的]研究AFLP标记在蔷薇遗传多态性方面的应用和5种不同蔷薇品种的遗传背景。[方法]应用10条人工设计的与接头序列相识别的AFLP选择性引物,用PstⅠ酶切,对5种蔷薇基因组DNA进行AFLP反应,分析不同蔷薇间的遗传相似系数和遗传距离。[结果]获得了108个AFLP标记,单引物获得的标记数在4～16,5种蔷薇的遗传相似系数为0.824(0.732～0.947),遗传距离为0.053～0.268。[结论]该研究为评价蔷薇的遗传稳定性提供了相关的参数。
[Objective] The research aimed to study the application of AFLP markers in detecting genetic polymorphic loci in 5 strains rose and their genetic background, [Method] Ten human-designed AFLP selective primers different from adaptor sequence were used, genomic DNA from 5 strains rose were restrictive by Pst Ⅰ , And their Genetic similarity coefficient and genetic distance were studied. [Result] The 108 AFLP markers were obtained, and the obtaining marker numbers of individual primer were 4-16, The similarity index of population in five strains rose was 0.824 （0.732-0,947）, and the genetic distance were 0.053-0.268, [Conclusion] AFLP technique was an effective method in genetic polymorphism monitoring of rose.
出处
《安徽农业科学》
CAS
北大核心
2008年第17期7143-7144,7209,共3页
Journal of Anhui Agricultural Sciences
基金
重庆文理学院基金(2005SK38
2007SK45)资助
关键词
蔷薇
遗传多态性
遗传相似系数
AFLP
Rose
Genetic polymorphism
Genetic similarity coefficient
AFLP（amplified fragment length polymorphism）
