摘要
目的:观察23例恶性肿瘤患者外周血淋巴细胞在体外经扩增培养后总CD3+、CD3+CD4+、CD3+CD8+淋巴细胞及CD3-CD16+56+(NK细胞)、CD3+CD16+56+(NKT细胞)的变化,初步观察淋巴细胞体外扩增培养后回输患者的生物安全性。方法:体外大规模诱导和扩增恶性肿瘤患者外周血淋巴细胞及单个核细胞,然后应用流式细胞术测定扩增前后CD3+、CD3+CD4+、CD3+CD8+、CD3-CD16+56+、CD3+CD16+56+细胞在淋巴细胞中所占百分比的变化。结果:CD3+、CD3+CD8+、CD3+CD16+56+细胞比例较培养前明显增加(P<0.01),而CD3+CD4+和CD3-CD16+56+细胞比例则明显的降低(P<0.01)。所有患者细胞回输治疗后无明显的毒副反应。结论:在本研究体系下体外诱导扩增培养肿瘤患者自体外周血NK细胞及致敏淋巴细胞效率较高,细胞毒性细胞比例增高较大,具有良好的生物安全性。
Objective ： To study the changes of CD3 ^＋ , CD3 ^＋ CD4 ^＋ , CD3 ^＋ CD8^＋ , CD3^-CD16 ＋ 56 ^＋ （ NK cell）, CD3 ^＋ CD16 ＋ 56 ^＋ （NKT cell）cells after amplification of 23 malignant tumor patients from peripheral blood in vitro, observing the biologic security of the lymphocyte after amplification in vitro and transfusion to the patients. Methods ： Inducing and amplifying the lymphocyte and mononuclear cells from peripheral blood of malignant tumor patients, using the flow cytometry to detect the percentage of CD3 ^＋ , CD3^＋ CD4^＋ , CD3^＋CD8^＋, CD3^-CD16 ＋56^＋ , CD3^＋ CD16 ＋56^＋ cells in lymphocyte, and then comparing their percentage before the expansion in vitro. Results： After cell culture, the percentage of CD3^＋ , CD3 ^＋ CD8 ^＋ , CD3 ^＋ CD16 ＋ 56^＋ cells increased greatly （P 〈 0.01 ）, and the percentage of CD3 ^＋ CD4 ^＋ and CD3^-CD16 ＋ 56 ^＋cells decreased obviously （P 〈 0.01 ）. There were no obvious side reactions on patients after transfusion. Conclusion： The induction and enlarged proliferation efficiency of NK cells and sensitized lymphocyte from malignant tumor patients＇ peripheral blood under this culture system is pretty high, the percentage of cytotoxic cells in lymphocyte increases greatly, and lymphocyte after proliferation are of good biologic security.
出处
《军医进修学院学报》
CAS
北大核心
2008年第3期184-186,共3页
Academic Journal of Pla Postgraduate Medical School
关键词
淋巴细胞亚群
体外扩增
免疫治疗
过继
流式细胞术
lymphocyte subgroups
In vitro expansion
immunotherapy, adoptive
flow cytometry
