摘要
利用过表达基因技术将从水稻中分离克隆的RACK1基因定向克隆至植物中间表达载体pCAMBIA1301中,构建了RACK1过表达融合基因植物表达载体pCAMBIA1301/R,通过根癌农杆菌EHA105将其导入水稻基因组。对获得的抗性植株的报告基因、基因组PCR及Southern Blotting进行检测分析。结果表明,该过表达基因已整合到水稻基因组中。
The paper aimed at establishing an experimental system for RACK1 gene function study and providing the intermidiate material for further studying the RACK1 gene function in rice. Utilizing over-expression technology,the RACK1 gene cloned from leaves of rice was inserted directly into the plant intermediate expression vector pCAMBIA1301, from which an over-expression fusion gene and a plant expression vector pCAMBIA1301/R were constructed. Genetic transform- ation to rice was mediated by EHA105. Transgenic assays were performed using GUS report,PCR and Southern Blotting. Result showed that the over-expression fusion RACK1 gene had been integrated into rice genome.
出处
《河南农业科学》
CSCD
北大核心
2008年第6期18-22,共5页
Journal of Henan Agricultural Sciences
基金
国家自然科学基金资助项目(30571120)
关键词
水稻
RACK1
植物表达载体
转基因植株
Rice （Oryza sativa subsp. ）
RACK1
Plant expression vector
Transgenic plants
