摘要
目的：研究慢性肾功能衰竭（ＣＲＦ）患者免疫功能紊乱的效应体ＣＤ２３分子的表达与调控。方法：利用细胞培养和间接免疫荧光技术，比较了ＣＲＦ患者与正常人外周血Ｂ细胞膜ＣＤ２３的表达，并研究重组人白介素４（ｒｈＩｌ－４）和重组人γ干扰素（ｒＨＩＦＮ－γ）对ＣＤ２３的调控作用。结果：ＣＲＦ患者外周血ＣＤ２３＋Ｂ细胞较正常人显著增高；ＩＬ－４能促进ＣＲＦ患者和正常人Ｂ细胞ＣＤ２３的表达；ＩＦＮ－γ可显著抑制ＩＬ－４诱导的Ｂ细胞ＣＤ２３的表达。结论：ＣＲＦ患者存在Ｂ细胞的激活；ＣＲＦ患者和正常人一样，外周血Ｂ细胞ＣＤ２３表达受ＩＬ－４上调和ＩＦＮ－γ下调影响；ＣＤ２３分子不仅是ＣＲＦ患者免疫功能紊乱的效应体，而且可能参与这些患者免疫紊乱的发病机理。
OBJECTIVE To study the expression of CD23 antigen and its regulation in patients with chronic renal failure(CRF).METHODOLOGY CD23 was investigated on peripheral blood B lymphocyte from CRF patients and normal donors by indirect immunofluorescence assay, and the influence of rhIL4 and rhIFNγ on its expression was examined.RESULTS (1) The CD23 expression was significantly higher on the B cells from CRF patients (48.81±12.57%) than on the B cells of healthy donors (the control)(16.20±5.08%)(P<0.001); (2) rhIL4 increased CD23 expression on the control (from 16.39±5.09% to 39.29±8.10%, P<0.05) and the CRF B cells(from 59.12±7.94% to 75.35±9.98%, P<0.05); (3) rhIFNγ specifically inhibited the rhIL4 mediated induction of CD23 expression on the control (from 39.29±8.10% to 18.0±3.31%, P<0.05) and on the CRF B cells (from 75.35±9.98% to 30.38±7.89%, P<0.001).CONCLUSION Uremic patients may have a B cell activation state in basal conditions. CD23 expression on B cell of CRF patients was upregulated by IL4 and downregulated by IFNγ. CD23 may not only be an effector of immune dysregulation in CRF, but also act as a costimulatory factor in the T cell activation associated with chronic uremia.
出处
《肾脏病与透析肾移植杂志》
CAS
CSCD
1997年第6期546-549,共4页
Chinese Journal of Nephrology,Dialysis & Transplantation
