摘要
为了研究CorA的镁离子转运机理,对大肠杆菌CorA的间质结构域区进行了删除突变分析,并利用酵母突变体互补技术进行了突变体活性测定。以酿酒酵母质膜镁离子转运系统敲除突变体菌株CM66及其基因型对照菌株CM52为宿主菌,建立了大肠杆菌CorA转运活性测定体系。结果显示:大肠杆菌CorA间质结构域N-端起始的24个残基对于CorA在酵母质膜中的表达或维持其本身正确构象发挥重要作用,CorA M124到D154段的部分残基在CorA介导的镁离子转运过程中起到重要作用。
Deletion analysis of the periplasmic domain of Escherichia coli CorA was used to investigate the magnesium transport mechanism of CorA. An E. coli CorA transport activity assay platform was developed using the yeast mutant complement method. The host strains were CM66, a Saccharomyces cerevisiae strain with its plasma membrane magnesium transporter removed and CM52 which was the genotype control strain. The deletion results show that the N-terminal 24 residues of the CorA periplasmic domain are important for maintaining the proper structure of CorA and regulating CorA expression in yeast plasma membranes. Some of the residues between M124 to DTM have a very important impact on CorA mediation of the Mg2＋ uptake process. This work discribes the role of the CorA periplasmic domain in the magnesium transport process and gives the detail functions of various sections of PPD during this process.
出处
《清华大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2008年第6期1057-1061,共5页
Journal of Tsinghua University(Science and Technology)
基金
国家自然科学基金资助项目(30340420442,30330160)
国家“九七三”重点基础研究项目(2004CB720005)
