摘要
目的:观察肺岩宁方对人肺腺癌细胞A549裸鼠移植瘤中缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)表达的丝氨酸苏氨酸蛋白激酶(Akt)信号调节的影响。方法:32只A549荷瘤裸鼠随机分为4组:模型组、顺铂组、肺岩宁方组和综合治疗(顺铂加肺岩宁方)组,每组8只。化疗组和综合治疗组于第1、3、5天腹腔注射顺铂各0.1mg,肺岩宁方组和综合治疗组予肺岩宁方0.4ml灌胃,持续21d。观察抑瘤率,逆转录聚合酶链反应(reverse transcription polymerase chain reac-tion,RT-PCR)检测移植瘤中HIF-1α mRNA表达,免疫组化法检测移植瘤中HIF-1α和磷酸化Akt(phos-phorylated Akt,p-Akt)蛋白表达。结果:肺岩宁方组裸鼠抑瘤率为34.17%,综合治疗组为64.38%。RT-PCR结果显示,综合治疗组HIF-1α mRNA的表达水平低于模型组(P<0.05)。免疫组化实验显示,化疗组、肺岩宁方组和综合治疗组移植瘤HIF-1α蛋白表达均明显低于模型组(P<0.01);肺岩宁方组和综合治疗组p-Akt蛋白表达明显低于模型组(P<0.05)。结论:益气养精法为主的肺岩宁方可抑制肺癌肿瘤生长,并与顺铂有协同作用。肺岩方对HIF-1α蛋白表达的抑制作用可能与下调p-Akt表达有关。
Objective： To observe the effects of Feiyanning Decoction, a compound traditional Chinese herbal medicine, in inhibiting the growth of transplanted A549 tumors in nude mice and the expressions of p-Akt and HIF-1α in A549 tumors. Methods： Thirty-two nude mice were transplanted with human lung adenocarcinoma cells （A549 cells） to make the mice model, and then randomly divided into four groups： untreated group （n = 8）, diamminedichloroplatinum （DDP） group （n=8）, Feiyanning group （n=8） and DDP plus Feiyanning group （n=8）. The nude mice in DDP group and DDP plus Feiyanning group were treated with DDP 0. 1 mg by peritoneal injection on the 1st, 3rd and 5th day after model establishment. The nude mice in the Feiyanning group and the DDP plus Feiyanning group were treated with Feiyanning Decoction for 21 days. Reverse transcription polymerase chain reaction （RT-PCR） was used to detect the expression of HIF-1α mRNA in tumors, and immunohistochemical method was used to detect the expressions of p-Akt and HIF-1α proteins in tumors. Results： The inhibition rates of tumor in Feiyanning group and DDP plus Feiyanning group were 34.17% and 64.38% respectively. RT-PCR showed that the expression level of HIF-1α mRNA in DDP plus Feiyanning group was lower than that in the untreated group （P〈0.05）. immunohistochemical staining showed that the optical density （OD） indexes of HIF-1α in DDP group, Feiyanning group and DDP plus Feiyanning group were lower than that in the untreated group （P〈0.01）. The expressions of p-Akt in the Feiyanning group and DDP plus Feiyanning group were lower than that in the untreated group either （P〈0.05）. Conclusion： Feiyanning Decoction is effective in inhibiting the growth of lung cancer. And the effect of inhibiting the expression of HIF-1α protein by Feiyanning Decoction may correlate with down-regulation of p-Akt.
出处
《中西医结合学报》
CAS
2008年第6期616-620,共5页
Journal of Chinese Integrative Medicine
基金
上海市重点学科建设资助项目(No.Y0302)
关键词
益气养精
肺岩宁方
人肺腺癌A549
缺氧诱导因子1
Α亚基
丝氨酸苏氨酸蛋白激酶
裸鼠
reinforcing qi and nourishing jing
Feiyanning Decoction
human lung adenocarcinoma cell strain
hypoxia inducible factor 1, alpha subunit
Akt
nude mice
