摘要
目的探讨外伤后细菌性致死性肉芽肿病原菌一痤疮丙酸杆菌对小胶质细胞(MG)增殖、凋亡的影响。方法病原菌以不同浓度和不同时间作用于小胶质细胞,采用甲基噻唑基四唑(MTT)法检测细胞活性,流式细胞仪检测细胞凋亡和细胞周期,并用光镜和电镜观察细胞形态学变化。结果经痤疮丙酸杆菌作用后小胶质细胞活性明显受到抑制,呈时间依赖性,结果具有统计学意义(P<0.05)。痤疮丙酸杆菌可诱导小胶质细胞的凋亡,凋亡率在实验组和对照组之间存在显著差异(P<0.05),但细胞周期检测发现实验组与对照组无明显差别。结论外伤后细菌性致死性肉芽肿病原菌-痤疮丙酸杆菌可抑制小胶质细胞的生长,诱导小胶质细胞的调亡,可能和患者的病原菌入颅后导致的炎症反应密切相关。
Objective To investigate the effect of propionibacteriumacnes （P. acnes ）, which is the pathogen of fatal bacterial granuloma after trauma, on the proliferation and apoptosis of microglia （MG）. Methods The microglia were treated with different concentrations of pionibacteriumacnes （ P. acnes） for different duration and they were observed by microscope and electronmicroscope. Activities were examined by methyl thiazolyl tetrazalium assay （MTT） and cell cycle or apoptosis was examined by flow cytometry. Results After treated with propionibacteriumacnes, the viability of microglia was obviously inhibited with a manner of time-dependent. There is significant difference between experiment and control group （P 〈 0. 05 ）. Propionibacterium acnes （ P. aches） could induce the apoptosis of microglia, and there was significant difference in apoptosis ratio between experiment and control group （P 〈0.05）. However, no significant difference between experiment and control group in cell cycle was found. Conclusion Propionibacteriumacnes （P. acnes）, which is the pathogen of fatal bacterial granuloma after trauma can inhibit the proliferation and induce the apoptosis of microglia. It might be closely related with the inflammatory reaction that is induced by pathogenic bacteria of patient which invaded the skull.
出处
《中华神经外科疾病研究杂志》
CAS
2008年第3期236-240,共5页
Chinese Journal of Neurosurgical Disease Research
关键词
痤疮丙酸杆菌
小胶质细胞
增殖
凋亡
Propionibacterium acnes
Microglia
Multiplication
Apoptosis
