摘要
目的:探讨黄体生成素(LH)类似物对离体大鼠颌下腺细胞分泌神经生长因子(NGF)的影响。方法:体外孵育大鼠颌下腺细胞并给予不同浓度的LH类似物,采用酶联免疫分析(ELISA)方法检测上清液中神经生长因子的含量。结果:当加入终浓度为10-6、10-4、10-2U/L的LH类似物孵育颌下腺细胞时,NGF的分泌量随着浓度的升高而逐渐增高;当LH类似物浓度为10-2、100、102U/L时,NGF的分泌量随LH类似物浓度的递减而降低;同时随着时间的增加,NGF的分泌量也会增加,孵育8h时达高峰,随后开始下降。结论:在体外LH类似物可双项调节大鼠颌下腺细胞分泌NGF的功能。
AIM： To investigate the effects of luteinizing hormone （LH） analogs on the secretion of nerve growth factor （NGF） in submandibular gland cells of rats in vitro. METHODS： The submandibular gland cells of rats were incubated in vitro and LH analogs at various concentration were added to the medium. The never growth factor （NGF） was detected by enzyme-link immunoassay （ELISA）. RESULTS： When LH analogs were at the concentration of 10^-6, 10^-4, 10^-2 U/L respectively, the secretion of NGF was gradually increased. However, when LH analogs were at the concentration of 10^-2, 10^0, 10^2 U/L respectively, the secretion of NGF was gradually decreased. When LH analogs （10^-5 IU/mL） were incubated at different time, the secretion of NGF was different. NGF reached the peak value when it was incubated for 8 h and then decreased gradually. CONCLUSION： LH analogs may regulate the secretion of NGF in the submaxillary gland cells of rats.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2008年第7期676-678,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
海军总医院院内基金资助(2007-2009)
