摘要
报道了鸡肌腺苷酸激酶基因的克隆和在温控启动子PRPL 调控下在大肠杆菌中的可溶性高效表达 .SDS PAGE分析表明 ,鸡肌腺苷酸激酶的含量可占大肠杆菌细胞总蛋白含量的 38% .利用John son等的干冰 /乙醇 冰水浴反复冻融法 ,可将此重组蛋白进行富集 ,纯度可达 85%以上 .
Chicken muscle adenylate kinase was overproduced in soluble form under the transcriptional control of the bacterial phage lambda P RP L promoters regulated by temperature sensitive repressors. SDS PAGE analysis demon strated that the enzyme was produced to the extent of as much as 38% of the total cellular protein. The soluble recombinant enzyme can be released from bacterial cells and enriched to purity of more than 85% by the Johnsons freeze/thaw method. Mouse anti rabbit muscle adenylate kinase monoclonal antibody can strongly crossreact with the recombinant enzyme .
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1997年第6期525-528,共4页
Progress In Biochemistry and Biophysics
