摘要
目的探讨子痫前期患者胎盘组织中TAC3基因的表达以及其影响因素。方法分别收集同期分娩的正常晚期妊娠和足月子痫前期患者的胎盘标本各12例,取胎盘母面绒毛小叶,进行细胞培养后,分别实时定量PCR检测胎盘的TAC3基因表达,以及由缺氧和氧化应激引发的TAC3基因在胎盘表达的改变,并用单向方差分析与t检验比较2组的差异。结果①对照组及子痫前期组的足月胎盘中均检测到TAC3基因。2组足月胎盘TAC3mRNA在病例组高水平表达,为对照组的1.7倍,TACR3mRNA在2组间差异无显著性。②低氧环境(2%氧气)细胞滋养层TAC3mRNA的表达显著低于正常含氧环境生长的胎盘细胞滋养层(21%氧气)1.8倍。而在氧化或低氧应激下的合胞体滋养层TAC3的表达差异无显著性。同样条件下TACR3的表达也差异无显著性。结论TAC3表达的主要位点是胎盘,其表达在人子痫前期足月胎盘中极高,但低氧和氧化应激在体外不诱导TAC3的表达。
[Objective] To study the expression of TAC3 gene in the placenta of patients who were in their eclampsism and its influential factors. [Method] 24 placentas were collected from the people who delivered during the same period. 12 of them were from normal women who were in their late pregnancy, the others from patients who were in full term and eciampsism. Cotyledons were taken and cultured. Then the expression of TAC3 gene was detected by real time polymerase chain reaction. It was also detected in cases of hypoxia and oxidative stress. They were compared by one way F test and t test. [Result] ①TAC3 gene was found in both groups and the expression of TAC3mRNA in patients was 1.7 times that of control group but with no significant deviation between the two groups. ②The expression of TAC3 mRNA in the cytotrophoblasts which were cultured in hypoxia was significantly lower than in the cytotrophoblasts which were cultured in normal condition. But the expression of TAC3 and TACR3 in syntrophoblasts which were cultured in oxidation stress and hypoxia had no significant deviation. [Conclusion] Placenta is the major locus of TAC3. The expression of TAC3 in the full term placenta of the patients who develop PE is significantly high. But the expression of TAC3 is not induced by hypoxia and oxidative stress in vitro.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2008年第11期1537-1540,共4页
China Journal of Modern Medicine
基金
湖北省十堰市科技局(No:2005ZD014)
