摘要
目的:观察基质金属蛋白酶-7(MMP-7)蛋白在鼻咽癌中的表达及与肿瘤侵袭转移的关系。方法:采用成组设计的对照试验方法,运用免疫组化及逆转录聚合酶链式反应(RT-PCR)技术,检测MMP-7蛋白及mRNA在鼻咽癌及慢性鼻咽炎组织中的表达。使用方差分析、q检验、t检验和X2检验进行组间差异的统计学分析。结果:MMP-7蛋白在鼻咽癌中的阳性表达率为79.17%,明显高于在慢性鼻咽炎中的表达率11.11%(P<0.001);MMP-7mRNA在鼻咽癌中的阳性表达率为89.58%,表达量为0.467±0.199,显著高于在慢性鼻咽炎组织中的表达率16.67%(P<0.001);MMP-7蛋白及mRNA的表达与鼻咽癌患者的性别、年龄无关,与鼻咽癌的T分期、N分期及临床分期密切相关(P<0.05)。结论:MMP-7在鼻咽癌中阳性表达率高,其表达随着肿瘤浸润深度、区域淋巴结转移范围和临床分期的增加而增强,与鼻咽癌侵袭及转移密切相关。
Objective:To observe the expression of matrix metalloproteinase - 7 ( MMP - 7) in nasopharyngeal carcinoma and it's significance in metastasis. Methods:Control trial was adopted in the experiment design. Immunochemistry and RT - PCR were used to detect the expression of MMP -7 in naso- pharyngeal carcinoma and chronic nasophar- yngitis. Anova analysis, Student - Newman -Keuhs test,t test and Chisquare test were applied in statistic analysis. Results:The expressive rate of MMP -7 protein in nasopharyngeal carcinoma were 79. 17%, which was obviously higher than that of chronic nasopharyngitis ( 11. 11% ). The expressive rate of MMP-7 mRNA in nasopharyngeal carcinoma was 89. 58% , which level was 0.467 ±0. 199, it was obviously higher than that of chronic nasopharyngitis (16.67%). There were no significant difference between the expression of MMP -7 and MMP - 13 and patients' sex and age, but referent to cancer invasive depth, sectional limp node metastasis and clinical staging. Conclusion: MMP-7 expresses in nasopharyngeal carcinoma, and the expression strengthens with the increasing of cancer invasive depth, sectional limp node metastasis and clinical staging. MMP-7 relates with nasopharyngeal carcinoma invasion and metastasis.
关键词
鼻咽癌
肿瘤侵袭与转移
基质蛋白酶-7
Nasopharyngeal Carcinoma
In-vasion and Metastasis
Matrix Metalloprotei-nase-7