摘要
本文目的是用原代培养的神经细胞探讨知母皂甙元（ＺＭＳ）对神经细胞Ｍ受体的调节作用。主要方法是：用原代培养的神经细胞，用Ｍ受体非选择性拮抗剂３Ｈ－ＱＮＢ结合分析法测定Ｍ受体密度，并以Ｍ受体不可逆阻断剂苯甲基偶酰基胆碱氮芥（ＢＣＭ）为工具，测定Ｍ受体代谢动力学参数。主要结果：向不同培养天数（９～１５天）的神经细胞加入ＺＭＳ（０．１ｍｍｏｌＬ－１），作用４８小时后对Ｍ受体密度均有显著的上调作用（Ｐ＜０．０１）。各种浓度的ＺＭＳ（０．１～１００ｕｍｏｌＬ－１）对３Ｈ－ＱＮＢ的结合均无明显抑制作用。用（０．１ｍｍｏｌＬ－１）的ＺＭＳ显著升高Ｍ受体的合成速率（Ｐ＜０．０１），而对降解速率常数无影响（Ｐ＞０．０５）。结论：ＺＭＳ能使培养９～１５天的原代配养的大鼠神经细胞的Ｍ受体密度上调，这种作用和激动剂或拮抗剂不同，并非ＺＭＳ直接作用于Ｍ受体结合位点所引起，而是由于ＺＭＳ能促进Ｍ受体的生成。离体培养的环境相对衡定，可以排除神经体液的间接作用。
Aim: To study the regulatory effects of ZMS, a Sapogenin from Zhimu (Rhizoma Anemarrhenae) on the neuronal muscarinic cholinergic receptors of primary culture of neonatal rat brain. Method:\ muscarinic antagonist 3H QNB was used to determine the receptor and the metabolic kinetic parameters were obtained by using the irreversible muscarinic blockade Benzilyl choline mustrad (BCM). Results:\ After being added to the culture medium for 48 hours, ZMS (0.1 mmol·L-1) significantly upregulated muscarinic receptor densities in different culture period ( P <0.01). No marked inhibition of 3H QNB binding was found when the range of ZMS was from 0.1￣100 umol·L-1. ZMS significantly elevated the production rate of neuronal muscarinic receptors ( P<0.01 ), with no influence on dissociation rate constant ( P>0.05 ). Conclusion:\ ZMS can significantly evelate the density of M cholinergic recptors in primary cultured rat brain cells. Such an action is not due to the direct binding of ZMS to the cells like agonists or antagonists. Instead, it is due to an stimulatory action of ZMS on the synthesis of M receptors
出处
《中国中医基础医学杂志》
CAS
CSCD
1997年第6期15-17,共3页
JOURNAL OF BASIC CHINESE MEDICINE
基金
国家自然科学基金
关键词
中药
知母皂甙元
原代培养
神经细胞
M受体
\ Sapogenin
Zhimu (Rhizoma Anemarrhenae)
Neuronal cells
\ Muscarinic receptsor.
