摘要
目的研究PARP-1、CK7／20、p53在Barrett食管和食管腺癌中的表达并探讨其临床意义。方法应用免疫组化法测定40例Barrett食管，28例食管腺癌和40例贲门黏膜组织中PARP-1、CK7／20、p53蛋白的表达。结果PARP-1在贲门黏膜、Barrett食管和食管腺癌中均有阳性表达，但在食管腺癌中的阳性率明显高于其他两组（P〈0．01）；CK7／20在肠化上皮细胞胞质、部分贲门柱状上皮和腺癌上皮细胞质内呈阳性表达；而p53在3组中均有阳性表达，且食管腺癌中阳性率明显高于其他两组（P〈0．05）；PARP-1与p53表达在BE组存在明显相关性（r=0．49，P〈0．01）。结论CK7／20可作为诊断肠上皮化生的敏感指标，但特异性欠佳。PARP-1和p53与Barrett食管及食管腺癌有关，检测PARP-1和p53对判断Barrett食管癌变风险有一定价值。
Objective To investigate the expression of poly （ADP-ribose） polymerase-1 （PARP- 1 ） , cytokeratins （CK） 7/20, and p53 in patients with Barrett＇s esophagus and esophageal adenocarcinoma, and to evaluate their significance. Methods Expression of PARP-1, CK7/20 and p53 were determined by immunohistochemistry in 108 patients （including 40 Barrett＇s esophagus, 28 esophageal adenocarcinoma and 40 cardiac mucosa）. Results The expression of PARP-1 was found in Barrett＇s esophagus, esophageal ade- nocarcinoma and cardiac epithelium with a significantly higher level in esophageal adenocarcinoma than the other two groups （P 〈 0. 01 ）. CK7/20 was expressed in much of intestinal metaplasia, part of cardiac epi- thelium and ad cells. The positive expression of p53 was observed in all three groups, and it was significantly higher in adenocarcinoma group than in other two groups （P 〈 0. 05 ）. PARP-1 expression is highly correlated with that of p53 in Barrett＇s esophagus （ r = 0.49, P 〈 0.01 ）. Conelusion CK7/20 is a sensitive but less specific indicator for intestinal metaplasia. Both PARP-1 and p53 are involved in the patho- genesis of esophageal adenocarcinoma and might help to determine the risk of Barrett＇s esophagus developing into esophageal adenocarcinoma.
出处
《中华消化内镜杂志》
2008年第6期304-308,共5页
Chinese Journal of Digestive Endoscopy
基金
人事部回国人员科技活动项目[（2005）129号]
