摘要
建立新的大鼠记忆障碍模型，观察强啡肽Ａ（１－１３）对局灶性脑缺血－再灌注后记忆障碍的影响。方法插线法建立大鼠左侧大脑中动脉阻塞（Ｌｅｆｔｍｉｄｄｌｅｃｅｒｅｂｒａｌａｒｔｅｒｙｏｃｃｌｕｓｉｏｎ，ＬＭＣＡＯ）缺血再灌注模型。步入型被动回避（暗环境被动回避）反应研究学习和记忆。学习训练前脑室注射运用不同剂量的κ阿片受体选择性激动剂强啡肽Ａ（１－１３）和κ受体拮抗剂ｎｏｒ－ｂｉｎａｌｔｏｒ－ｐｈｉｍｉｎｅ（ｎｏｒ－ＢＮＩ）研究对记忆的影响。结果暂时性ＬＭＣＡＯ造成了大鼠记忆障碍。０．３μｇ强啡肽Ａ（１－１３）能明显地改善遗忘现象，这一效应被ｎｏｒ－ＢＮＩ所阻断。结论暂时性ＬＭＣＡＯ引起的大鼠记忆障碍模型可靠、稳定。强啡肽Ａ（１－１３）通过κ受体介导作用，能够改善暂时性ＭＣＡＯ后的记忆障碍。
bjective Our study tried a new model of ischemiainduced amnesia in rats,and evaluated the effect of κopioidreceptor agonist dynorphinA(113) on the amnesia.Methods The transient focal cerebral ischemic model of LMCAO(left middle cerebral artery occlusion,LMCAO) was made by intraluminal suture.Learning and memory were investigated by stepthroughtype passive avoidance task.Intraventricular pretreatment of dynorphin A(113) and κopioidreceptor antagonist norbinaltrophimine (norBNI) before learning has been used to determine the effect on the postischemic disorder of memory.Results Rats memory had been injuried by transient LMCAO.Dynorphin A(113) in 0.3μg could significantly attenuate the ischemiainduced amnesia,and this protective effect of dynorphia A(113)may be antagonized by norBNI.Conclusion We conclude that the model of amnesia following transient focal cerebral ischemia was credible,and the study suggests that dynorphin A(113) can prevent the impairment of memory following transient focal ischemia,an event mediated by the activation of the κopioidreceptor.
出处
《中国行为医学科学》
CSCD
1997年第4期246-248,共3页
Chinese Journal of Behavioral Medical Science
基金
国家教委回国人员中心资助
关键词
脑缺血
记忆
强啡肽A
Cerebral ischemia Memory Dynorphin A(113)
