摘要
用聚丙烯酰胺凝胶电泳技术对荞麦属（Fagopyrum MiH）8个种（含大粒组7个种和小粒组1个种）33份荞麦资源的谷草转氨酶同工酶进行了研究。结果发现：谷草转氨酶同工酶酶带共14条。不同物种的酶带为3～5条，甜荞和苦荞分别有3带和5条带。酶谱分析及聚类分析表明：大粒组荞麦种的酶带与F.gracilipes等小粒组荞麦种间差异极大，甜荞和苦养酶带分别与F.megaspartanium和F.pilus相似，并分别与F.megaspartanium和Epilus聚类最近，暗示F.megaspartanium和F.pilus可能分别是甜荞和苦荞的祖先种。
The glutamate oxaloacetate transaminase isozyme of thirty-three accessions of cultivated and wild buckwheat belonging to eight species of genus Fagopyrum including seven species of the big-achene group and one species of the small-achene group were studied by means of polyacrylamide gel eletrophoresis （PAGE）. The results showed that there are a total of fourteen bands of glutamate oxaloacetate transaminase Isozyme, with a range from three bands to five bands among different buckwheat species. F. esculentum and F. tataricum re- spectively have a total of three bands and five bands among different accessions. The results of the zymograph analysis and the clustering showed that there are much great difference of zymographs between the big-achene group and the small-achene group and that F. megaspartanium and F. pilus are close in clustering tree to F. esculentum and F. tataricum, respectively, so, we support Chen＇ s hypothesis that F. megaspartanium and F. pilus may be the ancestor species of F. esculentum and F. tataricum, respectively.
出处
《种子》
CSCD
北大核心
2008年第5期39-42,46,共5页
Seed
基金
国家自然基金项目(30471116)
教育部新世纪人才支持计划(NECT2004-0931)
国家“十一·五”科技支撑计划(2006BAD02B06)
关键词
甜荞
苦荞
野荞
谷草转氨酶同工酶
系统关系
起源与进化
common buckwheat
tartary buckwheat
wild buckwheat
glutamate oxaloacetate transaminase Isozyme
phylogenetic relationship
origin and evolution
