摘要
目的探讨生脉解毒通络胶囊对单核细胞趋化蛋白-1(MCP-1)mRNA在糖尿病(DM)大鼠心肌中表达的影响及相关机制。方法链脲佐菌素诱导建立糖尿病模型,成模2周后分别灌服依那普利、生脉解毒通络胶囊高、中、低剂量,连续12周,于第14周末应用放射免疫法检测心肌局部血管紧张素Ⅱ(AngⅡ)含量,并应用RT-PCR技术检测MCP-1 mRNA的表达。结果与正常组对照,糖尿病模型组心肌局部AngⅡ含量及MCP-1 mRNA的表达均明显增加(P<0.01),依那普利及生脉解毒通络胶囊高、中剂量组可使糖尿病大鼠心肌局部AngⅡ含量及MCP-1 mRNA的表达下降(P<0.01、P<0.05),低剂量组无显著效果。结论持续血糖增高能导致实验性糖尿病大鼠心肌局部AngⅡ含量及MCP-1 mRNA表达增加,生脉解毒通络胶囊能在一定程度上抑制大鼠心肌MCP-1mRNA表达,其机制可能与减少心肌局部AngⅡ含量有关。
Objective To investigate the effects of Shengmaijiedutongluo Capsules（SJT Capsules） on monocyte chemoattractant protein 1（MCP-1） mRNA expression in myocardial tissue of Streptozotocin（STZ） induced experimental diabetic rats and its mechanism.Methods Rats were made into diabetes model by intraperitoneal administration of STZ.Two weeks after modeling,Model rats were administrated orally with Enalapril and SJT Capsules of large,median and small dose respectively for successive 12 weeks.Level of myocardial angiotensinⅡ（AngⅡ） was detected by immunoradiometry and MCP-1 mRNA expression in myocardial tissue was assayed by RT-PCR.Results The levels of myocardial AngⅡand MCP-1 mRNA expression in the model rats were higher than those in the normal controls（P〈 0.01）.In Enalapril and SJT Capsules of large, median dose groups,levels of AngⅡand MCP-1mRNA expression were lower compared with those in the model rats（P〈0.01、P〈0.05）,and no significant effects were found in the low dose group.Conclusion Long lasted hyperglycemia can lead to the over-expression of MCP-1mRNA in the myocardial tissue of diabetic rats.SJT Capsules may partly suppress it and decrease significantly the myocardial injury of diabetic rats by reducing the level of myocardial AngⅡ.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2008年第5期329-331,共3页
Journal of Beijing University of Traditional Chinese Medicine
基金
吉林省科技厅资助项目(No.2005-028)
