摘要
目的:观察苯甲酸雌二醇对γ射线诱导小鼠骨髓造血细胞凋亡的影响,以探讨其抗放射作用机制。方法:昆明种小鼠,随机分为正常组、单纯照射组和用药照射组3组,每组各15只。单纯照射组小鼠用7射线照射4.0Gy;用药照射组于照射前10d每只小鼠肌内注射0.1mg苯甲酸雌二醇,同时用γ射线照射4.0Gy;正常对照组不作任何处理。采用DNA琼脂糖凝胶电泳检测骨髓造血细胞DNA裂解片段,采用流式细胞术观察各组小鼠照射后4h、8h、12h骨髓造血细胞凋亡、Fas和Bcl-2表达的变化情况。结果:γ射线照后8h,骨髓造血细胞DNA裂解片段增加,出现了典型DNA"梯状"条带,而用药照射组和正常对照组均未见明显"梯状"条带。照射后4h、8h、12h,用药照射组骨髓造血细胞凋亡率均明显低于单纯照射组(P<0.01);Fas表达较正常对照组略有增加,并未出现单纯照射组所呈现典型的高峰期,与单纯照射组比较显著降低(P<0.01);Bcl-2表达不降反而升高,与单纯照射组相比差异显著(P<0.01)。结论:苯甲酸雌二醇可能是通过下调Fas和上调Bcl-2的表达抑制了γ射线诱导的小鼠骨髓造血细胞凋亡。
Objective：To investigate the effects of estradiol on ^60 Co γ-ray induced apoptosis of bone marrow hematopoietic cells of mice,and to discuss the related anti-irradiation mechanism. Methods： KM mice were randomly divided into 3 groups （15 mice/each group）： control group （without radiation）, pure radiation group and estradiol ＋ radiation group （ER group）. The pure radiation group was irradiated by 4.0 Gy γ-ray at a dose rate of 1.15Gy/min; the ER group was administered with 0.1 mg estradiol （IM） at 10 days before 4.0 Gy γ-ray radiation; and the control group received no special treatment. The apoptotic DNA segments of bone marrow hematopoietic cells were analyzed by DNA agarose gel electrophoresis; flow cytometry was used to examine the apoptosis rate of cells and expression of Fas and Bcl-2 at 4 h,8 h,and 12 h after irradiation. Results： Eight hours after radiation,the apoptotic DNA segments were obviously increased and apoptotic DNA ladder appeared, which was not seen in the other 2 groups. The apoptosis rate of bone marrow hematopoietic cells in ER group was significantly lower than that in the pure radiation group at 4,8, and 12 h after irradiation（P〈0.01）. Fas expression was only slightly elevated in the ER group than in the control group, but was markedly lower than that in pure radiation group（P〈0.01）, and showed no typical crest-time; Bcl-2 expression was significantly higher in the ER group than in the pure radiation group（P〈0.01）. Conclusion： Estradiol can decrease the apoptosis of mice bone marrow hematopoietic cells induced by γ-ray,probably through down-regulation of Fas and up-regulation of Bcl-2 expression.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2008年第6期648-650,共3页
Academic Journal of Second Military Medical University
