摘要
以水稻为材料,通过60Coγ射线辐射诱变,结合组织培养技术,采用以黑龙江省优势稻瘟病菌谱系产生的混合粗毒素提取液作为选择压力,进行继代培养和分化培养筛选抗瘟水稻突变体。研究表明,水稻成熟胚经过60Coγ射线辐照后,获得的愈伤组织诱导率很低而且褐化率很高,但是通过苗瘟抗性鉴定发现,再生植株中对稻瘟病表现抗性的植株比例较大,说明辐射诱发突变与生物技术相结合的抗病育种途径具有较大的应用潜力和较好的发展前景。
Rice seeds were irradiated with ^60Co γ-rays to induce mutants, which resistant to rice blast were screened in subculture medium and differentiation medium under mix toxin from dominant strains of Pyricularia grisea in Heilongjiang. The results indicated that the inductivity of callus of mature rice embryo by irradiation was very low and the browning rate of callus was very high. However, the resistance rate of resistant regenerating plants was obviously higher, which indicated the resistant breeding by irradiation and biotechnology had a great potential and a better prospect.
出处
《核农学报》
CAS
CSCD
北大核心
2008年第3期248-252,275,共6页
Journal of Nuclear Agricultural Sciences
基金
农业部引进国际先进农业科学技术计划(“948”计划)(2006-G61)
关键词
稻瘟病
粗毒素
组织培养
辐射诱变育种
抗瘟突变体
rice blast
phytotoxin
tissue culture
mutation breeding by irradiation
resistant mutant
