摘要
用线性扫描伏安法、循环伏安法及光谱法研究了尼古丁与DNA在0.2 mol/LpH 6.0的H2C2O4缓冲溶液中的相互作用。研究结果显示,随着DNA的加入,尼古丁峰电流降低,峰电位正移,说明尼古丁是以嵌入形式与DNA结合,生成了一种结合比为2∶1的非电活性化合物,结合常数β为1.56×109。并用多种电化学方法求得了电极过程的动力学参数。
The interaction between nicotine and DNA was studied in 0.2mol/L oxalic acid buffer solution （pH=6.0） by linear sweep voltammetry（LSV）, cyclic voltammetry （CV） and ultraviolet-visible （UV- Vis） spectroscopy. The results showed that the peak current decreased and the peak potentials shifted positively with the addition of DNA. Under the experiment selected conditions, the nicotine reacted with DNA to form a 2 ： 1 non electroactive complex with combining constant of 1. 56 × 10^9. The dynamics parameters of electrode process were investigated by various electrochemical techniques.
出处
《分析科学学报》
CAS
CSCD
2008年第3期291-294,共4页
Journal of Analytical Science
基金
国家自然科学基金(20775073)
河南省科技攻关项目(No.062440053)
