摘要
运用SYBR Green I荧光定量RT-PCR方法和放射免疫方法(RIA)分析了外源性雌二醇(estradiol-17β,E2)对异育银鲫脑垂体-甲状腺轴的影响。实验设计时间为10周,实验组每2周注射一次0.5mg.kg-1体重的E2(用57%酒精溶解),对照组注射同剂量的57%酒精。实验期间没有投喂饲料。在实验条件下,SYBR Green I荧光定量RT-PCR分析结果显示,注射3和5次外源性E2后,促甲状腺激素β亚基mRNA的表达水平与对照组相比均未有显著变化,但随着注射次数的增加,总体呈现升高的趋势。另一方面,RIA结果显示,注射3次外源性E2后,血清中甲状腺激素T3和T4含量水平与对照组相比均显著升高(P<0.05);注射5次后,与对照群相比差异极显著(P<0.01)。以上结果提示,外源性E2影响异育银鲫的正常生理状态。
In this study, SYBR Green I fluorescence quantitative reverse transcription polymerase chain reaction and radi Y （RIA） methods were used to investigate the effects of exogenous estradiol-17β （E2） on pituitary-thyroid axis of Carassius auratus. The study was carried out in 10 weeks during which E2 was injected every two weeks for experiment groups at a dose of 0. 5 mg per kilogram body weight （resolved in 57% ethanol）, while the same dose of 57% ethanol was injected for control groups. The fish were not fed throughout the experimental period. The results showed that compared with control group, thyroid-stimulating hormone β subunit mRNA levels did not have significant changes after both 3 and 5 times of E2 injection, but have an increasing trend. On the other hand, RIA showed that after 3 times of E2 injection, thyroid hormone T3 and T4 concentration levels increased significantly compared with control group （P 〈 0. 05 ） ; while after 5 times of E2 injection, T3 and T4 levels showed extremely significant differences （P 〈 0. 01 ） . All of the above demonstrated that exogenous E2 could influence the normal physiological functions of C. auratus.
出处
《南方水产》
2007年第1期31-36,共6页
South China Fisheries Science
基金
上海市重点学科建设项目(Y1101)
上海市教委发展基金项目(科04-135)
回国留学人员科研启动基金项目(科01-113)
