摘要
以23个油茶无性系叶片的DNA作为实验材料,从60个随机引物中筛选出10个最优引物,并扩增出102条谱带,其中多态性谱带70条,多态性比率为68.6%,谱带大小为200～2000bp。Shannon遗传信息指数为0.4793,平均观察等位基因数和有效等位基因数分别为2.000和1.519;遗传距离为0.1542～0.6931,油茶无性系之间具有较高多态性。运用平均聚类法分析23个油茶无性系的亲缘关系及多样性,在遗传距离为0.392时,23个油茶无性系聚为4类:湘林156、湘林210和湘林4号等18个油茶无性系为第1类;岑软2号和湘林97号为第2类;湘林34号和湘林11为第3类;湘林81号单独为1类。其中,湘林4号与湘林29的亲缘关系最近,湘林34与湘林78的亲缘关系最远。在此基础上,分析了油茶ISSR聚类结果与油茶农艺学性状的关系,从而为油茶优良无性系甄别、亲缘关系分析以及种质资源利用,在分子水平上积累了有效证据。
The genomic DNA extracted from tender leaves of 23 elite clones of Camellia oleifera （ Native species for edible oil in China） was used to perform ISSR （inter-simple sequence repeat） analysis. Of the 60 primers screened, 10 pieces of DNA fingerprint profiles containing 102 bands with 70 distinct polymorphic bands （68.6%） were obtained, and each primer produced 3 to 10 bands with the band size ranging from 200 bp to 2 000 bp. Result indicated that Shannon＇ s information index was 0.4793, and the mean observed number of alleles and the mean effective number of alleles were 2.0000 and 1.5185, respectively. The genetic distance varied from 0. 154 2 to 0.693 1. There exists abundant polymorphism among these elite clones of C. oleifera. The UPGMA analysis result shows that the 23 elite clones of C. oleifera could be classified into 4 groups, namely, 18 elite clones （ including Xianglin 156, Xianglin 210, Xianglin 4, etc） belong to the first group, Jinruan 2 and Xianglin 97 to the second group, Xianglin 34 and Xianglin 11 to the third group, Xianglin 81 to the fourth group when the genetic distance is 0. 392. On the basis of these analyses, the relationship between the clustering result and the agricultural characters of C. oleifera was discussed, which provides some references for the identification of elite clones, phylogenetic analyses and the utilization of germplasm resources.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2008年第6期19-23,36,共6页
Journal of Northeast Forestry University
基金
国家林业局重点项目(200401)
国家科技部农业科技成果转化基金(05EFN214300199)
“十一五”国家科技支撑计划专题(2006BAD18B02)
关键词
ISSR
油茶
优良无性系
遗传多样性
Inter-simple sequence repeat
Camellia oleifera
Elite clones
Genetic diversities
