摘要
目的建立幽门螺杆菌（Hp）长期感染蒙古沙土鼠（简称沙鼠）腺胃的模型，并观察Hp长期感染引起的胃黏膜病理改变及其与致癌剂N-甲基-N’-硝基-N-亚硝基胍（MNNG）是否具有协同致损伤作用。方法健康雄性沙鼠90只随机分为4组：对照组（22只）、Hp组（24只）、MNNG组（20只）、Hp＋MNNG组（24只）。采用国际标准菌株NCTC11637灌胃，建立Hp长期感染沙鼠腺胃模型。并在接种Hp4周后在相应组给予MNNG灌胃。接种Hp后20周及40周时分两批处死动物Warthin—Starry银染判定Hp定植情况，HE染色观察沙鼠胃黏膜病理改变。结果（1）成功建立了Hp长期感染沙鼠腺胃的动物模型。（2）沙鼠胃黏膜病理学变化显示：20周时，对照组沙鼠胃黏膜腺体排列整齐，未见腺体萎缩、肠上皮化生、非典型增生等异常表现；Hp组3只出现腺体萎缩；MNNG组1只出现腺体萎缩；Hp＋MNNG组5只出现腺体萎缩，1只出现肠上皮化生。加周时，对照组胃黏膜病理未见异常表现；Hp组6只出现腺体萎缩，5只出现肠上皮化生，1只出现非典型增生；MNNG组5只出现腺体萎缩，2只出现肠上皮化生，无非典型增生发生；Hp＋MNNG组10只全部出现腺体萎缩，7只出现肠上皮化生，5只出现非典型增生。Hp＋MNNG组发生癌前病变的例数多于其他各组，由于时间尚短，暂未观察到胃癌发生。结论HpNCTC11637可以稳定的定植于沙鼠腺胃黏膜，并使之出现类似于人感染Hp后出现的各种病理变化；Hp与MNNG两者均可对胃黏膜造成损伤，两者同时作用会导致胃黏膜更严重的病理改变，两者存在协同致损伤作用。
Objective To establish a model of long-term infection with Helicobacter pylori （Hp） in Mongolian gerbil （Meriones unguiculatus）, and to investigate if Hp combined with N-methyl-N＇-nitro-N- nitrosoguanidine （MNNG） has a synergistic effect to induce gastric mucosa injury. To investigate pathological changes of gastric mucosa during long-term Hp infection in Mongolian gerbil model. Methods 90 healthy male Mongolian gerbils were randomly divided into 4 groups ： Hp group （ n = 24） undergoing gastric perfusion of Hp suspension of the line NCTC11637 in brain-heart infusion （BHI） 10s - 109 CFU/ml once a day for 10 days and then gastric perfusion of 1 ml normal saline （NS） once a day for 10 days since the 4th week after Hp perfusion, Hp ＋ MNNG group （ n = 24 ） undergoing gastric perfusion of Hp solution once a day for 10 days and then MNNG 1 ml （2 mg/ml） once a day for 10 days, MNNG group （n =20） undergoing gastric perfusion of BHI once a day for 10 days and then gastric perfusion of MNNC once a day for 10 day since the 4th week after BHI perfusion, and control group （n = 22） undergoing gastric perfusion of BHI once a day for 10 days and then gastric perfusion of NS again once a day for 10 day since the 4th week after the BHI perfusion. 4 and 8 weeks 1 gerbil from the control group and 2 gerbils from the Hp and Hp ＋ MNNG groups each were killed to observe the pathological changes and Hp colonization by liquid-based urease test and Warthin-Starry silver staining. 20 and 40 weeks after the Hp inoculation 10 gerbils from each group were killed to observe the pathology of the gastric mucosa. Results （ 1 ） A Mongolian gerbil model of long-term Hp infection was successfully established. （2） Hp induced the process progressing from normal gastric mucosa→ chronic atrophic gastritis → intestinal metaplasia → dysplasia. Until 40 weeks after Hp infection, the gastric mucosa of the control group remained normal. Twenty weeks after Hp infection 3 gerbils in the Hp group and
出处
《中华医学杂志》
CAS
CSCD
北大核心
2008年第22期1518-1522,共5页
National Medical Journal of China
关键词
螺杆菌
幽门
胃肿瘤
模型
动物
蒙古沙土鼠
Helicobacter pylori
Stomach neoplasms
Models, animal
Mongolian gerbil
