摘要
目的探讨慢性乙型肝炎患者外周血中淋巴细胞亚群与肝组织炎症活动度的关系。方法对36例慢性乙型肝炎患者行肝脏穿刺术，对炎症程度进行病理分级，同时应用流式细胞技术测定其外周血单个核细胞膜上相关CD分子的表达情况，并与22例健康对照组进行比较分析。结果15例G0～G2期患者外周血中CD4^＋CD45RO^＋CD45RA^-及CD8^＋CD45RA^＋CD62L^＋淋巴细胞与健康对照组相比具有统计学差异（P〈0．05）；14例G3期患者外周血中CD3^＋、CD3^＋CD8^＋、CD8^＋CD28^＋、CD8^＋HLA-DR^＋CD未及CD3^-CD19^＋淋巴细胞与健康对照组相比具有统计学差异（P〈0．05）；7例G4期患者外周血CD4^＋CD45RA^＋CD62L^＋及CD19^＋CD5^＋淋巴细胞与健康组相比具有统计学差异（P〈0．05）；三组间淋巴细胞数均无统计学差异。不同组别的部分淋巴细胞之间具有一定的相关关系。结论慢性乙型肝炎患者外周血中淋巴细胞亚群数量的改变与病情的轻重有一定的相关性；不同活动期的慢性乙型肝炎患者外周血淋巴细胞之间具有一定的相关关系。
Objective To explore the relationship in peripheral blood lymphocyte subsets and inflammation activity in fiver tissue in patients with chronic hepatitis B infection. Methods 36 liver tissues were procured by hepatic puncture leaded under B - ultrasonic from patients with chronic hepatitis B, and classified as various period by international standardization. The expressions of CD3 、CD4 and CD3s etc. in peripheral blood lymphocytes from 36 HBV patients and control group（22 healthy people） were analyzed by flow cytometry （FCM）, and analyzed the correlation with inflammation activity in fiver tissue. Results The level of CD4^＋CD45RO^＋ CD45RA^- and CD8^＋ CD45RA^＋ CD62L^＋ expressions in peripheral blood lympho- cytes from 15 patients with chronic hepatitis B（ GO ~ G2） were higher than that of control group （P 〈0. 05） ; The levels of CD3^＋ ,CD3^＋ CD8^＋ ,CD8^＋ CD28^＋ ,CD8^＋ HLA - DR^＋ CD38^＋ and CD3^- CD19^＋ expressions in peripheral blood lymphocytes from 14 patients with chronic hepatitis B（G3） were higher than that of control group （ P 〈 0. 05 ） ; The levels of CD4^＋ CD45 RA^＋ CD62 L^＋ expressions in peripheral blood lymphocytes from 7 patients with chronic hepatitis B（G4） were lower and the levels of CD19^＋ CD5^＋ expressions were higher than that of control group （ P 〈 0. 05）. There were no statistics differences in the quantities of peripheral blood lymphocyte subsets in G0-G2, G3 and G4. There were positive correlations between the levels of part of CD expressions in the groups. Conclusion There were partially correlation between the quantities of peripheral blood lymphocyte subsets and the condition of illness in patients with chronic hepatitis B infection. There were no significant differences in the quantities and partially correlation between the quantities of peripheral blood lymphocyte subsets in different active periods of the patients with chronic hepatitis B infection.
出处
《临床肝胆病杂志》
CAS
2008年第3期180-182,共3页
Journal of Clinical Hepatology
基金
2003年度南京市医学科技发展项(YKK0328)
2003年度江苏省社会发展指导性项目(BS2003601)
