摘要
目的:探讨睾丸扭转复位后附睾丙二醛(Methylenedioxyamphetamine,MDA)、总抗氧化物(Total antioxidants content,TAC)含量与时间变化及意义。方法:SD大鼠建立左侧睾丸扭转复位模型。48只大鼠分为对照组和实验组,实验组分为术后1、2、5、10和15天五组,每组各8只。硫代巴比妥法检测附睾MDA的含量,铁离子还原法检测TAC的含量。结果:扭转复位1天组扭转侧附睾组织MDA含量与对照组差异无统计学意义,2天、5天、10天组MDA含量较1天组显著升高,15天组MDA含量与1天组差异无统计学意义;1天组扭转侧附睾组织中TAC含量与对照组差异不显著,2天、5天、10天组TAC含量较1天组显著降低,15天组TAC含量与1天组差异不显著。结论:睾丸扭转复位后附睾组织中脂质过氧化反应先升后降,抗氧化物活力先降后升。提示氧自由基是引起附睾急性缺血再灌注损伤的重要因素之一。抗氧化物的使用对防止附睾发生缺血再灌注损伤有保护作用。
Objective：To investigate the time variation of Methylenedioxyamphetamine （MDA）,Total antioxidants content （TAC） after testicular torsion reposition and its significance. Methods：The animal model of left testicular torsion was made in SD rat. All 48 rats were divided into 6 groups according to time period： group A（1d）、B（2 d）、C（5 d）、D（10 d）、E（15 d） after operation and control group. The content of MDA and TAC were measured. Results： The difference of the content of MDA and TAC between group A and control group was of no significance. The difference of the content of MDA and TAC between group B and group A was very significant. The difference of the content of MDA and TAC between group C、D and group A is significant. The difference of the content of MDA and TAC between group E and group A is of no significance. Conclusion：The peroxidation of lipid in epididymis first upgrade and then decrease. The anti-oxidant of lipid in epididymis first decrease and then upgrade.
出处
《临床泌尿外科杂志》
2008年第5期389-390,394,共3页
Journal of Clinical Urology
关键词
丙二醛
总抗氧化物
附睾
睾丸扭转
复位
Methylenedioxyamphetamine
Total antioxidants content
Epididymis
Testicular torsion
Reposition
