摘要
目的:探讨蒙药肝乐康胶囊剂体外抗乙型肝炎病毒(HBV)的作用。方法:将蒙药肝乐康胶囊剂作用于HepG2.2.15细胞系,MTT法检测样品对HepG2.2.15细胞的毒性,ELISA法检测细胞上清中HBsAg、HBeAg的变化,实时荧光定量PCR法检测细胞与其上清中总HBVDNA含量,综合评价蒙药肝乐康胶囊剂体外抗HBV效果,并探讨其药理机制。结果:蒙药肝乐康胶囊剂对HepG2.2.15细胞毒性低,对细胞分泌HBsAg、HBeAg较弱的抑制作用,对细胞内HBVDNA拷贝数有一定的抑制作用。结论:蒙药肝乐胶囊剂在体外有一定的抗乙型肝炎病毒作用,可能是通过抑制HBVDNA,诱导感染HBV的HepG2.2.15细胞凋亡而发挥抗病毒活性。
Objective： To investigate the Traditional Mongolian Medicine Ganlekang Capsule on HBV in vitro. Methods ： The cytotoxicity of Traditional Mongolian Medicine Ganlekang Capsule on HepG2.2.15 cell was detected using MTT. The levels of HBsAg or HBeAg in the cultured supernatant of HepG2.2.15 were tested by ELISA. The content of total HBV DNA was quantified by Taqman real-time PCR, The methods are used to evaluate the inhibitory, effect on HBsAg , HBeAg and HBV DNA copy number in vitro, and to investigate the pharmaco- mechanisms. Results ： Traditional Mongolian Medicine Ganlekang Capsule has low cytotoxicity for HepG2.2. 15, and can inhibit the cell secretion of HBsAg, HBeAg and HBV copy number. Conclusion ：Traditional Mongolian Medicine Ganlekang Capsule is effective in inhibiting HBV in vitro,perhaps by inhibiting HBV copy and inducing apoptosis in HepG2.2.15 cell.
出处
《中华中医药学刊》
CAS
2008年第6期1229-1231,共3页
Chinese Archives of Traditional Chinese Medicine
基金
内蒙古自治区教育厅资助重大项目(ZD01107)
关键词
蒙药肝乐康胶囊
乙型肝炎病毒
体外实验
traditional mongolian medicine GanLekang capsule
HBV
test in vitro
