摘要
目的比较均相酶法直接测定与Friedewald公式计算法估算2型糖尿病人血清低密度脂蛋白胆固醇(LDL-C)的变异性并与健康对照组比较。方法分别用均相酶法直接测定和F公式计算糖尿病人和正常人血清LDL-C含量,配对t检验分别比较两种方法在两组人群中的差别。结果在糖尿病人中,当1.8mmol/L≤TG<5.81mmol/L时两种方法有显著差异(P<0.001),当TG<1.8时,两种方法所得LDL-C值差异无显著性(P=0.203)。健康人群中当1.80mmol/L≤TG<5.03mmol/L时,两种方法无显著性差异(P=0.844),TG≤1.8mmol/L时,两种方法有显著性差异(P<0.001)。结论Friedewald公式计算法在健康人群和糖尿病人LDL-C测定时应用条件不同,建议用均相酶法直接测定LDL-C更适合临床应用。
Objective To compare the variability of the homogenous enzymatic determination and calculated estimation by Friedewald Formula in Low Density Liportein Cholesterol （LDL -C ） detection in T2DM patients＇ serum, and the difference with the healthy controls. Methods LDL - C both in T2DM patients and healthy controls were determined by the homogenous enzymatic assay and calculated estimation of F formular respectively. T Test was used to detect the variability. Results In normal people, when 1.80mmol/L≤TG 〈5.03 mmol/L, there was no significant difference between direct results and estimations （ P = 0.844）. There was difference when TG 〈 1.8mmol/L （ P 〈 0. 001 ）. In grow T2DM, the average result of Friedewald Formula was higher than direct determination despite the level of TG. When 1.80mmol/L≤TG 〈 5.82mmol/L, there was significant difference between direct results and estimations （P 〈 0. 001 ）. While TG 〈 1.80mmol/L, there was no significant difference （P = 0. 203）. Conclusion Regarding the levels of TG, there are different methods for determination of LDL - C in of healthy people and T2DM patients. It is suggested that direct homogenous enzymatic determination of LDL - C is suitable for chnical purpose.
出处
《中国热带医学》
CAS
2008年第4期587-589,共3页
China Tropical Medicine
