摘要
对浓缩铀２３５Ｕ内照射人淋巴细胞白血病细胞株Ｍｏｌｔ－４细胞和巨噬细胞株Ａｎａ－１细胞诱发的细胞凋亡及其防护因子进行了研究。实验中估算了２３５Ｕ在不同阶段培养细胞中的辐射累积吸收剂量。通过透射电镜的形态观察，发现Ｍｏｌｔ－４和Ａｎａ－１两株免疫细胞在受２３５Ｕ内照射作用下，可诱发核断裂，核染质边聚，以及呈现膜包裹着的凋亡小体形成为特征的细胞凋亡。而蛋白质合成抑制剂ＣＨＸ和ＲＮＡ合成抑制剂ＡｃｔＤ可显著抑制由核素２３５Ｕ诱发免疫细胞ＤＮＡ链断裂的作用，从而呈现出明显的对２３５Ｕ内照射诱发免疫细胞凋亡的防护作用。
The apoptosis in Molt-4 cell (a human acute lymphoblastic leukemia cell line)and Ana-1 cell(a macrophage cell line) were studied after internal irradiation with enriched uranium 235U.The cumulative radiation absorption dose of 235U in cultural cells in different periods were calculated.The morphological changes which were observed by electron microscope indicated that Molt-4 and Ana-1 immune cells after incubation with 235U,displayed chromatin fragmentation,margination of condensed chromatin,as well as the membrane-bounded apoptotic body formation.The finding shows that 235U can significantly increase the DNA fragmentation in immune cells,which may be inhibited statistically by CHX and ActD.So both CHX and ActD show radioprotective effect on Molt-4 and Ana-1 cells irradiated by 235U.
出处
《中国辐射卫生》
1997年第4期193-195,共3页
Chinese Journal of Radiological Health
基金
国家自然科学基金
