摘要
利用实时荧光定量PCR技术,根据转基因大豆(Roundup ReadyTM)的外源基因35S启动子序列设计引物和TaqMan MGB探针,对大豆粉中Roundup ReadyTM大豆含量进行了定量检测,根据这个检测体系建立了35S启动子Ct值与样品中转基因成分数量之间的标准曲线和线性回归方程(相关系数r2:0.9942)。本研究设计的方法还可以应用到多组分的食品、饲料等加工产品,检测转基因成分的含量,并可作为转基因食品常规PCR定性检测方法。
According to the PCR primers and TaqMan MGB probe of exogenous 35S promotor, the quantitative detection of genetically modified soybean （ Roundup ReadyTM ） was established by real-time PCR technology. According to this detection system, the standard curve of Ct vs genetically modified organism quantity was generated and a linear regression equation was obtained （r^2：0. 9942）. The results demonstrated that this method could be used in quantificational detection of multiple organism food.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第3期140-142,共3页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
广东省科技计划资助项目(2004A20508001)
