摘要
[目的]以卵蛋白致敏的哮喘豚鼠为动物模型,观察中药咳喘方对哮喘豚鼠肺组织病理学变化的影响。[方法]正常雄性豚鼠60只,体质量(300±20)g,随机分为正常对照组、模型组、泼尼松组、咳喘方组,每组各15只。验组分别给予泼尼松、咳喘方水煎剂(黄芩、地龙、杏仁、麻黄、当归、细辛、半夏等),连续灌胃给药14d,观察实验动物肺组织病理学变化。[结果]对照组肺及支气管结构正常,各级支气管上皮完整,肺泡间隔正常,少量炎细胞浸润。模型组支气管管腔狭窄,管壁增厚,黏膜皱襞增多,部分脱落;杯状细胞明显增加;气道壁大量嗜酸性粒细胞浸润;肺泡间隔增宽,其中可见嗜酸性粒细胞;部分肺泡腔变小。泼尼松组支气管黏膜皱襞增多,部分脱落,管壁轻度增厚,管腔轻度狭窄。咳喘方组支气管黏膜上皮轻度增厚,气道壁炎细胞浸润较轻,肺泡间隔明显变薄,肺泡腔趋于正常,其间有少量嗜酸性粒细胞浸润。[结论]中药咳喘方能显著改善哮喘豚鼠肺组织病理学变化,其作用优于泼尼松组。
[Objective] To observe the influence of Kechuan prescription on the pathological change of the lung tissue in the asthma guinea-pig induced by egg protein provocation test. [Methods] Sixty normal guinea-pigs with average weight of （300±20）g were provided from the experimental animal centre of Tianjin Medical University, They were randomly divided into four groups： normal control group, model group, prednisone group and kechuan prescription group. The asthma in guinea-pig was induced with Lvbaozhang＇s method. The experimental groups were administered with prednisone and kechuan prescription decoction respectively for 14 days. The pathological change of the lung tissue in the asthma guinea-pig was observed. [Results] The lung and bronchia of guinea-pigs in control group showed normal and intact bronchial epithelium. Alveolar septum was normal, and a small amounts of inflammatory cells infiltration was found. The bronchial lumens of guinea-pigs in model group were narrow, the bronchial wall was thickened, mucosal folds were increased, only part of mucosal folds was exfoliated; goblet cells were obviously increased, a lot of acidophilic granulocyte infiltration in the airway wall was seen. The alveolar septum was widened and there were acidophilic granulocyte in it; parts of alveolar space was shrink. The bronchial mucosal folds in prednisone group were increased, parts were exfoliated, bronchial wall was mildly thickened, and bronchial lumens were narrow. The bronchial epithelium in kechuan prescription group was mildly thickened, small amounts of inflammatory cells infiltration in the airway wall were found, alveolar septum was thinned, alveolar lumens tended to be normal, and there were small amounts of acidophilic granulocyte infiltration in it. [Conclusions] Kechuan prescription can obviously improve the pathological change of the lung tissue in the asthma guinea-pig, and its effect was superior than prednisone.
出处
《天津中医药》
CAS
2008年第3期217-219,共3页
Tianjin Journal of Traditional Chinese Medicine
关键词
咳喘方
哮喘豚鼠
病理学
Kechuan prescription
asthma guinea-pig
pathology
