摘要
海洋野生鱼与养殖鱼比较,其鱼油中含更多的二十碳五烯酸(EPA)、二十二碳六烯酸(DHA)、脂溶性维生素等活性成分。为提高海洋野生鱼的利用价值,以野生小带鱼为原料进行酶法提油工艺研究。分析了不同的温度,时间,pH值等影响因素下的提取、萃取以及离心效果,以响应面法确定了最佳的酶解工艺条件:液固比为6、pH7.3、酶量1000u/g原料、搅拌速度200r/min、45oC酶解90min;最优萃取条件:萃取剂100mL(每20g鱼糜原料)、pH4.0、40oC萃取25min;离心条件:离心速度3000r/min(1865g)、离心时间10min。上述工艺条件下提油率为79.90%。改进了传统的鱼油提取工艺,在活性成分保护上有较大改善。
Compared with cultured fish, the fish oil of ocean wild fish contains much more Eicosapntemacnioc acid （EPA）, Docosahexenoic acid （DHA）, fat-soluble vitamin. To improve the utility value of oacean wild fish, small hairtail was used as raw material to investigate the technology of extracting fish oil with enzyme. The variables to affect the efficiency of extraction, extracting and centrifugation were selected as temperature, reaction time and pH value. Optimal technology conditions were determined by the response surface method： The liquid/solid ratio is 6, pH 7.3, enzyme amount of 1000 trig raw material, agitation speed of 200 r/min, enzymolysis under 45℃ for 90 min. The optimum extraction conditions were as follows： 100 mL extractant （every 20 g surimi）, pH4.0, exrtacted under 40℃ for 25 min. The optimal centrifuge conditions were： centrifuge speed of 3000 r/min （1865 g）, centrifuged for 10 min. The oil extraction efficiency was 79.9%. This study developed the traditional technology of fish oil extraction, and improved the protection of the active components.
出处
《生物工程学报》
CAS
CSCD
北大核心
2008年第6期1022-1028,共7页
Chinese Journal of Biotechnology
基金
浙江省科技计划项目(No.001102219)资助~~
关键词
海洋野生鱼
鱼油
酶解
萃取
离心
工艺
ocean wild fish, fish oil, enzymolysis, extraction, centrifuge, technology
