摘要
目的:探讨3种不同方法检测维生素C清除自由基能力的差异,为选择一种有效评价抗氧化活性物质清除自由基能力的方法提供依据。方法:分别采用二苯代苦味肼基自由基(DPPH·)分光光度法、Mn2+-H2O2-PAR分光光度法和羟自由基(·OH)试剂盒法对不同浓度的维生素C清除自由基(·OH、DPPH·)的能力进行比较。结果:DPPH·分光光度法、Mn2+-H2O2-PAR分光光度法、·OH试剂盒法检测0.024mg/ml维生素C对自由基的清除率分别为80.09%、95.00%、9.56%,自由基清除率为50%时维生素C浓度(IC50值)分别为0.005、0.013、0.200mg/ml。结论:Mn2+-H2O2-PAR分光光度法的检测结果与DPPH·分光光度法基本一致,该方法可能可以作为评价抗氧化活性物质清除自由基能力的有效方法之一。
Objective： To investigate the difference between three different methods of detection the ability of vitamin C to eliminate free radicals and provide an evidence for the selection of an effective evaluation of antioxidant activity on scarvenging free radicals. Methods： The scavenging radical effects of vitamin C on ·OH, DPPH · were determined and compared by Mn^2＋ H2O2-PAR method,DPPH·method and hydroxyl radical reagent kit method, respectively. Results： In DPPH·method, Mn^2＋-H2O2-PAR method and hydroxyl radical reagent kit method, the scavenging radical effects of vitamin C in the concentration of 0.024 mg/mlwere80.09%0, 95.00%, 9.56% , the IC50 were 0.005, 0.013, 0.200 mg/ml, respectively. Conclusion： The results of Mn^2＋ -H2O2-PAR method and DPPH · method are consistent. Mn^2＋-H2O2- PAR method could be used to evaluate the scavenging radical activity of antioxidants.
出处
《新疆医科大学学报》
CAS
2008年第5期578-579,582,共3页
Journal of Xinjiang Medical University
