摘要
目的探讨乙型肝炎病毒前S1抗原(Pre-S1Ag)与乙型肝炎病毒DNA(HBV DNA)、e抗原(HBeAg)、抗HBe(anti-HBe)的相关性及临床价值。方法对848例乙肝患者血清用酶联免疫吸附法(ELISA)检测乙肝病毒血清标志物(HBV-M)、Pre-S1Ag,用荧光定量PCR测定HBV DNA、用速率法检测丙氨酸氨基转移酶(ALT)。同时对乙肝HBV-M全阴性的305例健康人检测血清Pre-S1Ag。结果848例乙肝患者中HBeAg阳性率为76.2%,Pre-S1Ag总阳性率为62.5%。其中,646例HBeAg阳性患者组中检出Pre-S1Ag阳性率62.2%;202例HBeAg阴性患者中检出Pre-S1Ag阳性率63.4%,两组的Pre-S1Ag阳性率无明显差异(P>0.05)。Pre-S1Ag阳性患者比Pre-S1Ag阴性患者的ALT异常率显著增高(P<0.01)。结论无论HBeAg阴性还是阳性,Pre-S1Ag同HBV的复制指标HBV DNA都有较好的一致性,是判断乙型病毒活动性的指标,是无条件开展HBV DNA检测时的最佳选择。
Objective： To investigate the correlation and clinical significance of hepatitis B virus Pre S1 antige （ PreS1Ag）, hepatitis B virus DNA （ HBV DNA）, hepatitis Be antigen （HBeAg） and anti - HBe. Method： For the 848 serum samples with HBV, the HBV serum markers （ HBV - M） and PreSIAg are detected by enzyme - linked immunosorbent assay （ELISA）, the HBV DNA is detected by florescence quantitative PCR, and alanine aminotransferase （ALT） is measured by rate assay. Results： The positive rate of HBeAg is 76. 2%, in 646 cases of HBeAg positive patients group, the positive rate of PreSIAg is 63. 4% in 202 cases of HBeAg negative patients group. The two groups have no significant difference （ P 〉 0. 05 ）. Abnormal ALT of the PreSIAg positiive patients is significantly increased than the PreSIA negative patients （ P 〈0. 01 ）. Conclusions： Regardless of negative or positive HBeAg, PreSIA has better consistency with HBV DNA. PreSIA is the judg- ment of hepatitis B virus activity indicators, and is the best choice unconditionally detecting HBV DNA.
出处
《湖北中医学院学报》
2008年第1期20-22,共3页
Journal of Hubei College of Traditional Chinese Medicine
