摘要
目的：研究灯盏细辛有效组分对体外培养鼠大脑皮层神经细胞存活的影响。方法：取出生1d内的乳鼠大脑皮层制成细胞悬液,接种于经多聚赖氨酸包被的96孔板中。于培养6d后,分别加入培养液及不同浓度的灯盏细辛提取物和灯盏乙素继续培养1d,采用MTT比色法测量其存活细胞的吸收值,同时行NSE检查。结果：NSE检查结果表明,培养6 d的存活细胞大部分均为神经元。与空白对照组比较,提取物浓度在0.1~1.6mg/ml,灯盏乙素高浓度时,存活细胞数均明显增加,且有一定的量效关系。结论：灯盏细辛两组分均能促进体外培养的大脑皮层神经细胞存活,且提取物活性较强。
Objective：To explore the effect of active components from Erigeron breviscapus （Vant.） Hand.-Mazz. on rat cerebral neurons in vitro. Methods： The cerebral cortex of post-natal under 1 day Sprague-Dawley rats were dissociated into cell suspension . The cell suspension was incubated in 96-well culture plates covered with polylysine in each well. Mter culturing for 6 days, the extractive and scutellarin were added to the cultures , continue to culture 1 days. Then, the OD of living cells in each well was measured by MTT assay. Some of the 6-day culture cells were stained with neuronal specific nolase （NSE） antibody . Results： Most of the living cells were cerebral neurons by NSE. Compared with control group ,the neuron survival in extractive group and high concentration of scutellarin were significantly higher than those in control group. Conclusions：The two active components from Erigeron bl＇eviscapus can all promote cerebral neurons to live in vitro, and the extractive did most.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2008年第2期35-37,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金资助项目,NO:30672602
关键词
灯盏细辛
有效组分
神经元
细胞培养
Erigeron breviscapus
active components
neuron
cell culture
