摘要
目的：建立蒙药清感九味丸的质量标准．方法：用高效液相色谱法对处方中草乌有效成分乌头碱进行定量分析，采用VP-0D6（150L×4．6）柱；流动相为甲醇一水-氯仿-三乙胺（70：30：1：0．2V／V）；流速为0．5mL·min^-1；检测波长为240hm．结果：乌头碱进样量在0．60～3．Opg范围内与峰面积线性关系良好（r=0．9997）．平均加样回收率为99．28％（CV％=0．99％）．结论：本试验方法既先进又简便，结果准确可靠，可作为该制剂中乌头碱的含量测定方法．
Objective： To establish the quality standard for Mongolian medicine Qingganjiuwei pill. Methods： Determining the aconitine content in Acontum Kusnezoffii by HPLC on the chro- matographic column of VP- ODS （150L × 4.6 ） and with methanol - water- chloroform - tri- ethylamine（70：30：1：0.2 V/V）as a mobile phase and flow rate of 0.5mL-min^-1. The detective wavelength was 240nm. Results：Obvious linear relation was showed between the input mass of a- conitine ranging from 0.60-- 0.30μg and the peak area（r = 0. 999 7）. The average rate of recovery for added sample was 99.28 % （CV% = 0.99 % ）. Conclusion： This method is not only simple but also advanced and the detection result is reliable. So it can be taken as a determination method to determine the content of aconitine in this preparation.
出处
《内蒙古民族大学学报（自然科学版）》
2008年第3期329-332,共4页
Journal of Inner Mongolia Minzu University：Natural Sciences
