摘要
在正己烷溶剂中脂肪酶Novozym 435可催化丁基苯酞的酸解和转酯化反应。对于酸解反应,可以用饱和盐溶液预平衡法或者直接加水合盐法来控制反应体系的水活度,最适水活度为0.62,相应的丁基苯酞的转化率为15.9%。对于转酯化反应,适宜的反应体系是加水合盐到反应体统中,最适水活度亦为0.62,相应的丁基苯酞的转化率为49.4%。而且Novozym435对外消旋的丁基苯酞具有拆分效果。
Lipase Novozym 435 could catalyze acidolysis and transesterification of 3-n-butylphthalide in hexane. The ways to control water activity by preequilibration with saturated salt solution and direct addition of salthydrate were all available for the acidolysis of 3-n-butylphthalide. The optimum water activity was 0.62 and corresponding conversion of 3-n-butylphthalide was 15.9% for the acidolysis. The suitable reaction system was direct addition of salthydrate to the reaction system of transesterification of 3-n-butylphthalide. The optimum aw was also 0.62 and corresponding conversion of 3-n-butylphthalide was 49.4% for the transesterification. Furthermore, resolution of racemic 3-n-butylphthalide could be catalyzed hy Novnzym 435.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2008年第4期64-67,共4页
Food and Fermentation Industries
基金
教育部长江学者和创新团队发展计划(编号：IRT0532)
关键词
丁基苯酞
Novozym
435
酸解
转酯化
水活度
3-n-butylphthalide, Novozym 435, acidolysis, transesterification, water activity
