摘要
过量耗氧(EPOC)是衡量动物无氧代谢能力的重要生理指标,本研究在25℃条件下对不同体重组鲇鱼Silurus asotus Linnaeus(<30g、30—50g、50—100g和>100g)力竭性运动后EPOC进行了测定,旨在揭示不同体重鱼类在EPOC方面的适应机制。实验结果显示:鲇鱼力竭性运动后耗氧率(VO2)峰值立即出现(2min),随后逐渐恢复到稳定状态水平。本研究根据实验数据统计分析结果,提出了力竭运动后鲇鱼VO2恢复过程的数学模型(VO2=a+be-ct),并对其中参数的生物学意义进行阐述。通过对各体重组过量耗氧参数(a、b和c)的分析比较,发现鲇鱼体重的增加使有氧代谢能力降低,无氧代谢的能力却有较大提高。
The elevated oxygen consumption following exhaustive exercise, termed ＂excess post-exercise oxygen consumption＂ （EPOC） can be used to assess the non-aerobic oxygen cost of exercise. To investigate the relationship between body weight and non-aerobic oxygen capacity,the effect of body weight （ 〈 30 g,30-50 g,50-100 g and 〉 100 g） on EPOC was study in Chinese catfish （Silurus asotus Linnaeus） at 25 ℃ using a open-flow respirometer designed by our laboratory. Resting oxygen consumption rates decreased significantly with the increase of body weight （ p 〈 0. 05 ） and the values were 3.32,2.84,2.60 and 2.26 mg O2/min· kg for 〈 30 g, 30-50 g, 50-100 g and 〉 100 g body weight groups, respectively. The oxygen consumption was immediately increased to peak value at 2min after exhaustive exercise and slowly decreased to a stabilized level. Peak oxygen consumption rates decreased significantly with the increase of body weight （p 〈 0. 05） and the values were 10.27,9.16,8.13 and 7.82 mg O2/min· kg for 〈 30 g, 30-50 g,50-100 g and 〉 100 g body size groups, respectively. The change of post-exercise oxygen consumption rate could be described as VO2 = a ＋ be^-ct .Where ‘a＇ could be considered as resting oxygen consumption, ‘b＇ as potential metabolic scope elicited by exhaustive exercise and ‘c＇ as a parameter for recovery rate of post-exercise oxygen consumption rate. The equations were VO2 = 4.20 ＋ 7.96e^- 0.133t （ n = 21, R^2 =0. 922, p 〈 0.001 ） for 〈 30g group, VO2 = 3.84 ＋ 6.68e^-0.094t （n= 15,R^2 =0.902,p 〈0.001） for 30-50g group, VO2=3.74＋5.68e^-0.089t（n = 16, R^2 =0.774,p 〈 0.001） for 50-100g group and VO2=3.41 ＋ 5.37e^-0.080t（n=7,R^2=0.803,p〈0.001） for〉 100g group. The values of ‘a＇ , ‘b＇ and ‘c＇ all decreased with the increase of body weight, but the EPOC had an increased tendency with the increased of body weight and calculated EPOC by above equations were 59.85,71.06,63.82 和 67.13 mg O2, respectively. The calculat
出处
《水生生物学报》
CAS
CSCD
北大核心
2008年第3期380-386,共7页
Acta Hydrobiologica Sinica
基金
国家自然科学基金(30700087)
重庆市自然科学基金资助(2007BB1226)资助
关键词
过量耗氧
力竭性运动
鲇鱼
Excess post-exercise oxygen consumption （EPOC）
Exhanstive exercise
Silurus asotus Linnaeus
