摘要
目的研究急性白血病患者细胞周期调控蛋白D1(Cyclin D1)不同水平的表达,探讨Cyclin Dl在急性白血病中的作用机制和临床意义.方法采用荧光实时定量RT-PCR方法检测51例急性白血病(AL)患者Cyclin D1 mRNA水平表达,良性血液病对照组16例.结果急性白血病组Cyclin D1表达明显高于对照组;急性髓系白血病(AML)与急性淋巴细胞白血病(ALL)组间Cyclin D1表达水平无显著差异性;难治复发组Cyclin D1表达水平最高,初发未治组次之,完全缓解组最低;初发未治组中随访的治疗后未缓解组Cyclin D1表达水平高于完全缓解组.结论Cyclin D1与临床病情进展程度有一定的关系,尤其与病情的复发关系较为密切,动态观察患者Cyclin D1水平变化可作为评价患者治疗效果及预后的指标之一.
Objective To determine the expression of Cyclin Di protein in patients with acute leukemia, and to investigate Cyclin D1＇s function and clinical significance in acute leukemia. Methods The expression of cyclin D1 mRNA was determined by real-time quantitative RT-PCR method in 51 patients with acute leukemia. Samples of benign hemopoietic patients were taken as normal control （16 cases）. Results The expression of Cyclin D1 in patients with acute leukemia was significantly higher than that in control group, There was no significant difference in the expression of Cyclin D1 between ALL and AML. The expression of Cyclin D1 was diverse among the three experiment groups ; the highest level was in the refractory or relapse group while the lowest level was in the newly diagnosed group. In the follow-up visit after treatment., the expression of Cyclin D1 in patients who hasn＇ t released was higher than that in patients of remission. Conclusion Cyclin D1 is correlated with progression of the disease in a certain degree, especially with relapse. Dynamic observation with the level of Cyclin D1 can be used to evaluate the treatment outcomes and act as a prognostic marker for acute leukemia.
出处
《昆明医学院学报》
2008年第2期21-24,共4页
Journal of Kunming Medical College
基金
云南省教育厅科学研究资助项目(5Z03150)
