摘要
BACKGROUND： The pathways induced/activated by mercury poisoning that lead to muscle pain remain unclear. The present study addressed the structural changes observed in the peripheral nerve following mercury poisoning. OBJECTIVE： To establish the mercury poison rat model, rats were intragastrically administered mercury, The correlation between post-mercury poison-induced muscular pain and tibial nerve morphological changes were observed. DESIGN： Observational contrast animal study. SETTING： Shangdong Academy of Occupational Health and Occupational Medicine. MATERIALS： Thirty adult Sprague Dawley rats of equal gender. Mercury chloride （HgCl2, analytical grade： 99.99%; batch number： 990402） was provided by Shanghai Chemical Reagent Factory, and sodium dimercaptopropanesulfonate （DMPS） injection by Shanghai Harvest Pharmaceutical Co., Ltd. （batch number： 0309011）. METHODS： This study was performed at the Animal Experimental Center of Shangdong Academy of Occupational Health and Occupational Medicine from December 2005 to January 2006. Rats were randomly divided into high-dose mercury, low-dose mercury, and control groups, with 10 rats in each group. Rats in the two mercury groups were intragastrically administered 17 mg/kg and 8.5 mg/kg HgCl2 solution, respectively, once a day to establish a rat model of subacute mercury poisoning. Rats in the control group were intragastrically administered 2 mL saline, once a day. Intragastric administration in the three groups lasted for （20± 2） days. After model establishment, rats in the two mercury groups were injected DMPS once a day to remove mercury. The injection lasted for 3 days after every 4-day interval. Seven days was regarded as one treatment cycle, and there were two treatment cycles in total. MAIN OUTCOME MEASURES： Mercury-induced muscular pain status; ultrastructural changes of the right tibial nerve following model establishment and mercury removal under transmission electron microscope. RESULTS： Thirty rats were included in
BACKGROUND： The pathways induced/activated by mercury poisoning that lead to muscle pain remain unclear. The present study addressed the structural changes observed in the peripheral nerve following mercury poisoning. OBJECTIVE： To establish the mercury poison rat model, rats were intragastrically administered mercury, The correlation between post-mercury poison-induced muscular pain and tibial nerve morphological changes were observed. DESIGN： Observational contrast animal study. SETTING： Shangdong Academy of Occupational Health and Occupational Medicine. MATERIALS： Thirty adult Sprague Dawley rats of equal gender. Mercury chloride （HgCl2, analytical grade： 99.99%; batch number： 990402） was provided by Shanghai Chemical Reagent Factory, and sodium dimercaptopropanesulfonate （DMPS） injection by Shanghai Harvest Pharmaceutical Co., Ltd. （batch number： 0309011）. METHODS： This study was performed at the Animal Experimental Center of Shangdong Academy of Occupational Health and Occupational Medicine from December 2005 to January 2006. Rats were randomly divided into high-dose mercury, low-dose mercury, and control groups, with 10 rats in each group. Rats in the two mercury groups were intragastrically administered 17 mg/kg and 8.5 mg/kg HgCl2 solution, respectively, once a day to establish a rat model of subacute mercury poisoning. Rats in the control group were intragastrically administered 2 mL saline, once a day. Intragastric administration in the three groups lasted for （20± 2） days. After model establishment, rats in the two mercury groups were injected DMPS once a day to remove mercury. The injection lasted for 3 days after every 4-day interval. Seven days was regarded as one treatment cycle, and there were two treatment cycles in total. MAIN OUTCOME MEASURES： Mercury-induced muscular pain status; ultrastructural changes of the right tibial nerve following model establishment and mercury removal under transmission electron microscope. RESULTS： Thirty rats were included in
基金
the grants from Shandong Medical Academy, No. 2004-023
