摘要
胶胞炭疽菌是引起红掌炭疽病的病原菌。根据GenBank中炭疽属不同种的ITS序列差异,设计了胶胞炭疽菌的特异性引物E1/E2,由此建立的PCR检测体系可以从38个胶胞炭疽菌菌株中扩增得到329 bp的特异性条带,而扩增其它近似或相关菌株时没有相应的特异性条带。该检测体系对胶胞炭疽菌基因组DNA的扩增灵敏度达到10 pg。将引物E1/E2与ITS区通用引物进行套式PCR扩增后,检测灵敏度至少提高10 000倍。当土中胶胞炭疽菌分生孢子达到200个/g土时可检测出。进一步利用此检测体系对携带病原菌的灌溉水、发病组织进行检测,均能快速稳定地检测出病原菌。
Colletotrichum gloeosporioides caused anthracnose in Anthurium andraeanum. Based on differences in internal transcribed space （ITS） sequences of Colletotrichum genus, a pair of species-specific primers, E1 and E2, was synthesized. The primers amplified a single PCR band of 329 bp with DNA extracted from C. gloeosporioides isolated from A. andraeanum, while other relative strains had no corresponding band. The detection sensitivity was 10 pg of genomic DNA. Using ITS1/ITS4 as the first round primers and E1/E2 as the second round primers, the detection sensitivity increased 10 000-fold to 10 fg. The detection sensitivity for the soil pathogens was 200 conidia/g soil. The PCR-based method developed here could stably and quickly detect the pathogen from water samples and diseased plant.
出处
《植物病理学报》
CAS
CSCD
北大核心
2008年第2期113-119,共7页
Acta Phytopathologica Sinica
基金
广州市2004年科技计划项目
关键词
胶胞炭疽菌
红掌
分子检测
套式PCR
Colletotrichum gloeosporioides
Anthurium andraeanum
molecular detection
nest PCR
