摘要
目的：建立牛奶中氯霉素（CAP）的GC-ECD分析方法。方法：牛奶中CAP经乙酸乙酯提取，用正己烷去除脂肪，硅胶柱纯化样品，BSTFA-TMCS试剂硅烷化衍生，用甲苯溶解后进GC-ECD分析。结果：实验结果表明，在线性范围0.005-2.5μg/ml内，相关系数r=0.9981，回归方程Y=36967.77X＋249.02，回收率为85.8%-94.5%，变异系数（CV）为1.7%-2.2%，最低检测限为1.0μg/kg。结论：本方法线性关系良好，回收率高，变异系数小，最低检测浓度低，应用本方法检测牛奶中氯霉素的残留量，基本上满足了灵敏、快速、准确可靠的分析方法要求。
Objective： To establish a method for the determination of chloramphenicol （CAP） in milk by GC - ECD analysis. Methods：CAP in milk was determined in toluene solution after being extracted by ethyl acetate. The fat was removed by n - hexane and the milk powder purified by silica gel column and silanized by BSTFA -TMCS. Results：The results showed that in the linear range of 0.005 to 2. 5 μg/ml, the correlation coefficient r = 0. 9981, the regression equation was Y = 36967.77X ＋ 249.02, the recovery was between 85.8% and 94. 5%, while the coefficient of variation （CV） between 1.7% - 2. 2%, the lowest detection limit was 1.0 μg/kg. Conclusion：With reliable producitivity, a wide linear range with good recovery, RSD, and a preferable low detection limit, the result revealed that this method is sensitive, rapid, accurate.
出处
《中国卫生检验杂志》
CAS
2008年第3期458-459,547,共3页
Chinese Journal of Health Laboratory Technology
关键词
衍生化
气相色谱法
牛奶
氯霉素
Derivatization
Gas chromatography （GC）
Milk
Chloramphenicol （CAP）
