摘要
为改善传统脱氮工艺中需要外加有机碳源从而增加运行费用的缺点,采用平板夹层培养和Hungate厌氧培养技术相结合的方法,从土壤中分离到一株能够同时利用硫化物和硝酸盐的化能自养型兼性厌氧菌3-1F.16S rDNA测序分析表明,该菌与红平红球菌的相似性达99%.菌株3-1F的代谢特征表现为接种后24 h后进入对数生长期,60 h达到生长高峰,此时,硫代硫酸盐和硝酸盐的利用率分别超过94%和75%,代谢产物以单质硫和氮气为主,含少量硫酸盐.目前,具备同步脱硫脱氮功能的红平红球菌尚未见报道,该菌株的获得为研究废水同步脱硫反硝化工艺并通过生物强化方式提高工艺的处理效能提供了微生物资源.
In order to improve the defects in traditional denitrification process that additional organic carbon increases the operational costs, a strain of facuhative anaerobic bacterium, 3-1 F, was isolated from soil using the method of double-layers plate and the Hungate anaerobic operation technique, for which, sulfide was adopted for its electron donor and nitrate for electron acceptor. Identification based on 16S rDNA sequence and physiological biochemical analysis indicates that the isolate has a homologous similarity of 99% with Rhodococcus erythropolis. The characterization of this strain shows that it can reach the logarithmic phase after inoculation for 24 h, and can reach a peak after inoculation for 60 h. Under these conditions, the thiosulfate and nitrate removal rates are over 94% and 75% , respectively. And the end products of this strain mainly consist of elemental sulfur and nitrogen gas, as well as small amount of sulfate. The achievement of strain 3-1F provides a usful microbiology resource for the efficiency improvement of simultaneous desulfurization and denitrification process through the way of bioaugmentation.
出处
《哈尔滨工业大学学报》
EI
CAS
CSCD
北大核心
2008年第4期536-539,共4页
Journal of Harbin Institute of Technology
基金
教育部新世纪优秀人才支持计划项目(2005800612)
关键词
同步脱氮脱硫
红平红球菌
鉴定
代谢特征
simultaneous desulfurization and denitrification
Rhodococcus erythropolis
identification
metabolic characteriazation
