摘要
为研究实验性变态反应性神经炎（Ｅｘｐｅｒｉｍｅｎｔａｌａｌｅｒｇｉｃｎｅｕｒｉｔｉｓ，ＥＮＡ）和格林－巴利综合征（ＧＢＳ）的发病机理，寻找ＧＢＳ的有效诊断、治疗方法，本实验采用反复超速离心、稀盐酸等电点沉淀、ＣＭ－２２弱酸性阳离子交换层析等方法，从牛硬脊膜内马尾神经根中提取到高纯度髓鞘碱性蛋白Ⅱ（ＭｙｅｌｉｎＢａｓｉｃＰｒｏｔｅｉｎⅡ，Ｐ２）。鉴定结果表明，该蛋白的分子量为１５０００，ｐｌ＝９．５，在ＳＤＳ－ＰＡＧＥ垂直板电泳、ＰＡＧＥ圆盘电泳中显示一条蛋白区带，在双向免疫扩散、微量免疫电泳中，与进口标准抗牛Ｐ２血清之间形成一条沉淀线，ＨＰＬＣ测定氨基酸成份与Ｋａｄ－ｌｕｂｏｗｓｋｉ报告的国外四个实验室测定的结果大致相同，具有诱导ＥＡＮ的生物活性。
To study the pathogenesis of experimental allergic neuritis (ENA)and Guillain Barre syndrome(GBS)and to find the effective methods to diagnose and treat GBS,myelin basic protein Ⅱ(P2)was isolated and purified from bovine intradural nervi coccygeus root by the acid extract、ultracentrifuge and exclusion chromatography with CM 22 column Results showed that the molecular weight was 15 000, and pI at pH 9 5 by isoelectric focu sing electrophoresis It migrated as a single band on SDS PAGE Double immunodiffusion and microimmunoelectrophoresis indicated the specificity of the P2 to standard P2 antiserum The amino acid composition of the P2 was the same as that of previously reported preparation by Kadlubowski et al It was highly neuritogenic to induce ENA in the rabbits
出处
《免疫学杂志》
CAS
CSCD
北大核心
1997年第4期239-242,共4页
Immunological Journal
关键词
髓鞘碱性蛋白Ⅱ
纯化
鉴定
G-B综合征
Myelin basic protein Ⅱ P2, Guillain Barre syndrome, Experimental allergic neuritis
