摘要
目的研究泉州地区孕妇风疹病毒的感染情况,为进一步诊断、预防和治疗提供科学的依据。方法应用实时荧光定量PCR技术对泉州地区800例孕妇血清中风疹病毒RNA(RV-RNA)进行量化的检测。结果检出阳性患者47例,阳性率为5.88%(47/800)。阳性患者的基因拷贝数均大于参考值(2.00%,每毫升拷贝数),患者每毫升拷贝数范围位于1.31×102～9.38×108之间。结论荧光定量PCR检测风疹病毒有其众多的优越性,这对正确诊断、判断预后、研究母婴传播、指导临床都具有重要的理论意义和使用价值。
Obkective：To investigate the infection of the pregnant wonman＇s Rubella Virus（RV）in Quanzhou in order to provide the reference for diagnosis,prevention and treatment of RV.Methods：To examine the 800 cases of pregnant women＇s RV in Quanzhou by uses of fluorescence quota PCR.Results：47 were positive in the total 800 cases,the positive rate is 5.88%.The copies of the positive cases were higher than the reference（2.00%,copies/ml）.The range of all the patients were between 1.13×10^2～9.38×10^8.Conclusion：Fluorescence quota PCR is a good technique in examining RV patients,it can be well used to diagnose,judge the restoration,and it is useful to study the female infant disseminates and direct the clinical practice.
出处
《中国优生与遗传杂志》
2008年第5期27-27,86,共2页
Chinese Journal of Birth Health & Heredity
