摘要
目的:观察抗内毒素Fab'对严重烧伤早期肠源性内毒素血症小鼠肠道损伤的保护作用,探讨防治烧伤脓毒症的新措施.方法:采用严重烧伤早期肠源性内毒素血症小鼠模型,分为对照组(A组)、烧伤组(B组)和治疗组(C组),分别于6、12、24和48h4个时相点测定血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-10(interleukin-10,IL-10)的含量;肠黏膜组织病理检测损伤情况.结果:与A组相比,烧伤后血清TNF-α、IL-1β和IL-10含量明显增高,24h达到高峰(TNF-α:242.06±3.93ng/Lvs14.98±1.13ng/L;IL-1β:37.86±5.88ng/Lvs14.39±2.43ng/L;IL-10:324.78±65.82ng/Lvs97.63±20.48ng/L,均P<0.01);C组TNF-α、IL-1β、IL-10含量,在6、12、24和48h4个时相点时(99.69±10.67ng/L,19.19±1.17ng/L,160.44±24.99ng/L;172.07±22.47ng/L,22.29±3.32ng/L,185.44±22.30ng/L;125.98±6.93ng/L,28.39±2.59ng/L,237.11±30.28ng/L;107.88±5.24ng/L,26.23±2.51ng/L,207.86±20.35ng/L)较烧伤组显著降低(均P<0.01).病理检查结果提示C组较B组肠黏膜损伤有不同程度减轻.结论:抗内毒素Fab'能抑制内毒素所诱导的TNF-α、IL-1β产生,同时调节血清中的IL-10水平,减轻内毒素对机体的损害,从而起到对严重烧伤后肠源性脓毒症的防治作用.
AIM： To investigate the protective effect of Fab’ against endotoxin on intestinal mucosa during the early stage in severely burned gutderived endotoxemia mice, and to explore a new method for preventing and curing burn sepsis. METHODS： The severely burned gut-derived endotoxemia mice model was used, and the mice were divided into 3 groups： control group （group A）, burn group （group B） and Fab＇ treatment group （group C）. At the 6th, 12th, 24th and 48th h after burn injury, the levels of serum tumor necrosis factor-α （TNF-α）, interleukin-1β（IL-1β） and interleukin-10 （IL-10） were determined by enzyme-linked immunosorbent assay. Intestinal tissues were collected for pathological examination. RESULTS： In comparison with those in group A, the levels of serum TNF-α, IL-1β and IL-10 in group B increased significantly and reached the peak 24 h after burn injury （TNF-α： 242.06 ± 3.93 ng/L vs 14.98 ± 1.13 ng/L; IL-1β： 37.86 ± 5.88 ng/L vs 14.39 ± 2.43 ng/L; IL-10： 324.78 ± 65.82 ng/L vs 97.63 ± 20.48 ng/L; all P 〈 0.01）. The levels of TNF-α, IL-1β and IL-10 at 6, 12, 24 and 48 h in group C （6 h： 99.69 ± 10.67, 19.19 ± 1.17, 160.44 ± 24.99 ng/L; 12 h： 172.07 ± 22.47, 22.29 ± 3.32, 185.44 ± 22.30 ng/L; 24 h： 125.98 ± 6.93, 28.39 ± 2.59, 237.11 ± 30.28 ng/L; 48 h： 107.88 ± 5.24, 26.23 ± 2.51, 207.86 ± 20.35 ng/L） were sig- nif icantly different from those in group B （all P 〈 0.05）. Pathological examination showed a lower degree of intestinal mucosal injury in group C than in compared with group B. CONCLUSION： The Fab’ against endotoxin can signifi cantly decrease the level of serum TNF-α, IL-1β and IL-10 during the early stage in severely burned gut-derived endotoxemia mice, and consequently alleviate the endotoxin-induced injury.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第13期1402-1406,共5页
World Chinese Journal of Digestology
基金
国家重点基础研究发展规划项目(973)
No.2005CB522601~~
