摘要
目的:评价精子运动参数在研究豚鼠精子超激活运动中的作用,进一步确定豚鼠精子超激活运动的指标。方法:采用计算机辅助精液分析(CASA)系统检测豚鼠精子不同孵育时间(0、1、3、5、7h)的运动参数。结果:豚鼠精子孵育过程中精子曲线运动速度(VCL)、平均路线速度(VAP)、头部侧摆幅度(ALH)随孵育时间的增加而提高,并在5h达到最大值,而直线运动速度(VSL)、直线性(LIN)、向前性(STR)、鞭打频率(BCF)随孵育时间的增加而降低,并在5h达到最低值。结论:豚鼠精子获能培养过程中在发生超激活运动前精子的运动方式可发生很大的变化。
Objective： To evaluate the role of sperm kinematic parameters in the hyperactivation of Guinea pig spermatozoa, and to confirm the index of their hyperactivated motility. Methods： Computer-aided sperm analysis （CASA） was used to describe the kinesis parameters of the Guinea pig spermatozoa incubated for 1,3, 5 and 7 hours. Results ： The curvilinear velocity, average path velocity and amplitude of lateral head displacement were increased with time and reached the peak at 5 hours, while the straight linear velocity, linearity, straightness and beat cross frequency were gradually decreased with time and hit the bottom at 5 hours. Conclusion ： The sperm movement pattern changes greatly before hyperactivation during the capacitation of Guinea pig spermatozoa.
出处
《中华男科学杂志》
CAS
CSCD
2008年第5期387-390,共4页
National Journal of Andrology
基金
国家自然科学基金(30471243)
关键词
超激活运动
精子
计算机辅助精液分析
hyperactivated motility
spermatozoa
computer-aided sperm analysis
